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Community supporting commercial building in Chuangke Town, Wenquan Town, Haidian District, Beijing
Beijing Baiao Innovation Technology Co., Ltd
Community supporting commercial building in Chuangke Town, Wenquan Town, Haidian District, Beijing
1Mouse tumor necrosis factorα(TNF-α)Elisatest kitProduct Introduction
·Item No.E-EL-M3063
·Alternative name:DIFTheTnfaTheTNF-aTheTNFSF2TheTnlg1fTheTnfsf1aTheTNFalphaTheTNF-alphaTheTnfTheCachectinTheTumor necrosis factorTheTumor necrosis factor ligand superfamily member 2
·Size:48TThe96TThe96T*5



·Reactivity: Mouse
·Detection range: 7.81-500 pg/mL
·Sensitivity: 4.69 pg/mL
Product Application |
ELISA |
Detection Principle |
This kit uses a double antibody sandwichELISAThe law. Using anti mouseTNF-αAntibody coated on an enzyme-linked immunosorbent assay (ELISA) plate, mice in the sample (or standard) during the experimentTNF-αIt will bind to the coated antibody. Then add the anti mouse agent with biotinylation in sequenceTNF-αAntibody and horseradish peroxidase labeled avidin, anti mouseTNF-αAntibodies and mice bound to coated antibodiesTNF-αCombining, the specific binding of biotin and avidin forms an immune complex, and the free components are washed away. Add chromogenic substrate(TMB),TMBUnder the catalysis of horseradish peroxidase, it appears blue and turns yellow after adding termination solution. Using an enzyme-linked immunosorbent assay (ELISA) reader450 nmMeasurement at wavelengthODValue,TNF-αConcentration andOD450The values are proportional to each other, and the sample can be calculated by plotting a standard curveTNF-αThe concentration. |
reaction type |
Sandwich-ELISA |
specification |
96 T/ 48 T/ 96 T × 5 |
reaction time |
3 h 30 min |
reactivity |
Mouse |
detection method |
colorimetry;ELISA;Sandwich method |
Testing Scope |
7.81-500 pg/mL |
sensitivity |
4.69 pg/mL |
Sample volume |
100 μL |
Sample Type |
Serum, plasma or other biological fluids |
specificity |
Mice in detectable samplesTNF-αAnd there is no significant cross reaction with other similar substances |
precision |
Both intra plate and inter plate coefficients of variation<10% |
recovery rate |
80%-120% |
Storage conditions |
2-8℃ |
Validity period |
12month |
Elabscience ElisaMouse tumor necrosis factorα(TNF-α)Elisatest kitAgent Beijing Baiao Innovation Technology Co., Ltd.:
(1) Radiation five major service directions:
1.Domestic distribution and agency of reagents and consumables;
2.Ordering of foreign reagents (direct order, or overseas agent);
3.Genome sequencing (second-generation, second-generation)+Three generations);
4.Genotyping(SNPDetection: Low/middle/middle High throughput projects;
5.Biological sample transportation (domestic);
(2) Multiple agency brands involved30Multiple well-known brands, operating agents500Multiple brands:
QiagenTheSigmaTheR&DTheEpigentekTheThermoFisherTheDNA GenotekTheUSA ScientificThe
MatreyaTheSage ScienceTheList LabsTheMacrocyclicsTheSteraloidsThe
NIBSCThemobioTheImmuoduxThecellsystemsThemedicagoTheabcam
CSTTheRocheTheBethylThesino BiologicalTheclodrosomeTheadi
BIO-WORLDTheTOKU-ETheHYPHEN BioMedTheMoltoxTheZeptoMetrix
International HumicThebiocytexTheToxin TechnologyTheStretch
ElastinThebiomaxTheOrflo TechnologiesTheAccegenTheAdvbiomartTheGenlantisThebiocolor
biocolorTheCygnus Technologies LLCTheEnzyme ResearchTheBio/Data Corporation
Greer LabTheSolarbyThebangslabsThebiolineThemusechemTheNovocastra
stemrdThehyphen-biomedThepolyplusTheMoltoxTheexiqonThepentapharmTheVerichemicallabs
GenStarTheALZETThelucernatechnologiesThemeridianTheoriginsThezimmerpeacocktech
horizondiscoveryTheReagentsThemegazymeTheelisasystems………
All biological reagents and consumables can be imported, especially obscure brands, which can be purchased by customers. Excellent price, short delivery time, can be expedited, fast10Delivery within days; Ensure service quality and provide after-sales service for all products. We provide cash on delivery services for most customers,
The company has established long-term cooperation with major enterprises, institutions, and research institutions based on excellent service and good reputation since its establishment. Our clients include Peking University, Tsinghua University, DiDa University, various institutes of the Chinese Academy of Sciences, major pharmaceutical companies, and so on.
Professional ordering of domestic and foreign reagents and consumables, with readily available stock and first-hand supply. Welcome to cooperate.
