Molecular Experiment Molecular Interaction: SPR Surface Plasmon Resonance

1、 Technical Introduction

Surface plasmon resonance（Surface Plasmon Resonance, SPR）It is a highly sensitive biosensing technology that provides real-time, label free binding kinetics and affinity data by monitoring the interactions of biomolecules on metal thin film surfaces, and is widely used in molecular interaction detection.

2、 Technical principles

The core of SPR technology is to use light to generate evanescent waves in different media and resonate with the plasma waves on the metal surface. When the ligand fixed on the chip and the analyte flowing through the chip bind and dissociateThe change in surface quality of the chip causes a change in resonance angle, and the sensor image will record the signal values under the interaction in real time. Based on this change curve, the dissociation constant (KD), binding rate constant (Ka), and dissociation rate constant (Kd) of intermolecular interactions can be obtained

3、 Application scenarios

1. Biomedical field: widely used in protein-protein interactions, protein-small molecule drug interactions, nucleic acid protein interactions, nucleic acid nucleic acid interactions, antibody antigen molecule affinity determination, etc. Through SPR technology, the binding process between biomolecules can be monitored in real-time and dynamically, and binding kinetic parameters can be obtained to gain a deeper understanding of the intermolecular interaction mechanisms.

2. In the field of drug research and development, it is applicable to drug screening, drug target interaction studies, and pharmacokinetic studies. By monitoring the affinity interaction between compounds and targets, lead compounds can be quickly screened, and effective kinetic data can be provided for drug structure-activity relationships.

3. Other fields such as surface plasmon resonance sensors, scanning near-field optical microscopy, thin film optics and thickness measurement, holographic imaging technology, * switches, precision angle measurement, etc. also have applications.

4、 Technical advantages

1. Real time dynamic monitoring: It can dynamically monitor the process of biological molecule interactions, obtain the reaction rate and kinetic equation of interactions.

2. The sample does not require labeling: it can maintain the properties and state of the detected molecule, while avoiding the labeling process and its adverse effects such as false positive signals.

3. Low sample demand: This belongs to non-destructive analysis methods, and the testing process will not cause any chemical or physical damage to the sample. Fast detection process: characterized by high throughput, high sensitivity, and high efficiency.

5、 Sample type

A solution containing the substance to be tested

6、 Sample requirements

50-1000ug (varies greatly depending on the substance being tested)

7、 Sample preparation&transportation

Sample stored at -80 ℃ and transported by dry ice

8、 Required reagents

Ligand molecules (can be tested for antibodies, proteins, nucleic acid sequences, etc.).

9、 Experimental cycle

The project requires personalized customization

10、 Other

Please provide detailed information about the substance to be tested, including the binding kinetics and affinity of protein-protein, protein nucleic acid, drug target, etc. The project is highly personalized and requires separate evaluation and quotation based on specific circumstances;

The control molecule may need to be purchased separately.