Millipore ultrafiltration tube inner tube 4ml Huicheng Biological in stock

Millipore ultrafiltration tube inner tube 4ml Huicheng Biological in stockUltrafiltration is the most effective method for desalination and concentration of large molecules(Space) ConvenientThe method has good repeatability and guaranteed yield, and standard procedures have been established in the preparation of samples such as proteins, antibodies, viruses, nucleic acids, and vesicles; It has also become the mainstream method in the current hot extracellular vesicle preparation. Amicon from Millipore ® Ultra ultrafiltration tubes use centrifugation to quickly process samples, which is a basic equipment used by many researchers every day.

Internal tube volume

Merk MILLIPORE ultrafiltration tubes are divided into three volumes: 0.5ml, 4ml, and 15ml.

molecular weight

There are five molecular weights: 3kd, 10kd, 30kd, 50kd, and 100kd.

purpose

MILLIPORE ultrafiltration tubes can be used for biological sample purification, protein concentration, desalination, and buffer replacement. The vertical structure of the membrane reduces concentration polarization, allowing for rapid centrifugation and direct absorption of recovered samples. The concentration factor can reach up to 80-100 times, making it a powerful assistant for sample processing.

Ordering information:

How to choose the right cut-off molecular weight?

Millipore's ultrafiltration tubes use its regenerated cellulose membrane, defined as: a determined NMWL ultrafiltration membrane should retain at least 90% or more of spherical molecules of this size, i.e. 100kDa spherical protein using 100k Ultracel ® The yield of regenerated cellulose membrane exceeds 90%.

1. The molecular shape of the sample is spherical

The first principle for selecting ultrafiltration tubes is that the membrane pore size should be smaller than the target molecule. If the molecule has an approximately spherical three-dimensional structure (rather than a clearly linear molecule), in the Millipore regenerated cellulose ultrafiltration membrane system, choosing an ultrafiltration tube with a cut-off molecular weight "equal to" or "slightly smaller" than the target molecule is the best choice. This operation is fast and good, ensuring sample recovery rate, shortening centrifugation time, and significantly reducing the risk of large molecule damage.

2. Sample without reference

Because the sample molecules have different shapes, in order to(Space) MaximizeFor sample recycling, for samples without reference, an ultrafiltration tube with a cut-off molecular weight of about 1/2 of the target molecular weight can be initially selected.

Taking the AU0.5 of 0.5mL as an example, the principle for selecting the cut-off molecular weight of other models is the same, but the specific centrifugal force and centrifugation time are different. )

How to Best(Space) Maximizerate of recovery?

Reverse centrifugation recovery can achieve the highest yield.

① The reverse centrifugation recovery design enables efficient recovery of protein samples, especially small volume samples, avoiding sample loss or operational errors caused by gun suction;

② 0.5mL, 2mL, and 70mL ultrafiltration tubes are designed for reverse recovery mode.

Can ultrafiltration tubes be used for desalination and concentration?

The use of ultrafiltration tubes for concentration and dialysis bags or desalination columns for desalination are experimental procedures that many laboratories have been using. In fact, using ultrafiltration tubes for "desalination+concentration" is also a small trick for many skilled protein operators. Using Millipore ultrafiltration tubes for desalination and concentration can reduce transfer errors and sample loss with fewer tools and shorter time, resulting in better desalination and concentration effects.

Millipore ultrafiltration tube inner tube 4ml Huicheng Biological in stock

Related Millipore ultrafiltration tube products:

Millipore/Millipore ultrafiltration tube inner tube 0.5ml molecular weight 3kd/10kd/30kd/50kd/100kd

Millipore/Millipore ultrafiltration tube inner tube 4ml molecular weight 3kd/10kd/30kd/50kd/100kd

Millipore/Millipore ultrafiltration tube inner tube 15ml molecular weight 3kd/10kd/30kd/50kd/100kd

Related recommendations:

Shanghai Huicheng Universal Consumables Universal Suction Head Micro Centrifugal Tube Needle Filter

Common biochemical consumables: Blue cap bottle, sampling tube, sealing film, freezing storage box, including sterile, DNA free, and RNA free containers

Shanghai Huicheng Biological Cell Culture Plate, Deep Hole Plate, PCR Plate, Enzyme linked immunosorbent assay Plate, with complete specifications and more favorable prices for whole boxes

Plastic centrifuge tube sold over 10000 units in Shanghai Huicheng, with a volume of 1.5ml and 10ml

Extra number! Extra number! Cytiva in stock has arrived!!!!

Universal pipette tip suitable for Thermo Eppendorf, Dalong Ruining, and UNV series

Millipore ultrafiltration tube UFC901096 UFC903096 UFC905096 UFC910096 outer tube 50ml inner tube 15ml 10kd 30kd 50kd 100kd

20ul compatible with Ruining pipette bag, 1000 suction tips per bag, 30 bags per box

20ul compatible Ruining pipette box with 96 suction tips per box, 50 boxes per box

20ul compatible with Ruining pipette box filter cartridge suction head 96 pieces/box, 50 boxes/box

20ul compatible with Ruining pipette stack box, 96 suction tips per box, 8 boxes per stack, 768 tips per stack, 20 packs per box

250ul compatible with Ruining pipette bag suction head 1000 pieces/pack, 20 packs/box

250ul compatible Ruining pipette box with 96 suction tips per box, 50 boxes per box

250ul compatible with Ruining pipette box filter cartridge suction head 96 pieces/box, 50 boxes/box

250ul compatible with Ruining pipette stack box, 96 suction tips per box, 9 boxes per stack, 864 tips per stack, 24 tips per box

1000ul compatible with Ruining pipette bag suction head 1000 pieces/pack, 10 packs/box

1000ul compatible with Ruining pipette box, 96 suction tips per box, 50 boxes per box

1000ul compatible with Ruining pipette box filter cartridge suction head 96 pieces/box, 50 boxes/box

1000ul compatible with Ruining pipette stack box, 96 suction tips per box, 5 boxes per stack, 480 tips per stack, 24 packs per box