Microfluidic flow cytometer

On-chip Droplet SelectorMicrofluidic single-cell sorting and dispensing device

The Changer of Filtering Rules

The On chip series instruments bring innovative technologies for cell culture/screening, contributing to the technological development in fields such as food and environment.

Droplet technology:

Droplet technology has gained widespread attention due to its significantly improved efficiency compared to traditional cell screening techniques. At present, droplet technology is widely used in research fields such as the cultivation of uncultured microorganisms, the development and screening of innovative drugs, and the acquisition of mutant cell lines.

On-chip Droplet SelectorMicrofluidic flow cytometerTechnical features:

Traditional cell sorting instruments cannot be used as sheath fluids due to the inability of oil solutions or droplets as samples to withstand impact, resulting in droplets being unable to be used as machine samples. It is very difficult to retest or separate encapsulated cell/microbial droplets separately.

On-chip Droplet SelectorBy utilizing microfluidic chip technology,Capable of sorting droplets at high speed and separating target droplets one by one, directly dispensing the separated individual droplets into the wells of the cell culture plate. The innovative screening method can significantly reduce the time and effort required compared to traditional screening methods, and significantly improve efficiency.

The On chip Droplet selector integrates "separation" and "dispensing" function modules, which can specifically handle droplet samples. By using air pressure to separate target droplets into individual droplets, non-destructive dispensing of one droplet per hole has been achieved with an accuracy of over 90%. The objects of analysis and separation are not limited to droplets, but also cells or GMD (gel droplets) can be detected.

On-chip Droplet SelectorMicrofluidic flow cytometerCore strengths：

1. Use microfluidic technology to separate target droplets and inject them individually into an orifice plate.

2. The entire process from sample analysis to separation is integrated onto a small-sized microfluidic chip.

3. The Flowshift method controls the sorting of samples through air pressure, with a sorting pressure as low as 0.3 PSI, achieving non-destructive sorting.

4. In sorting mode, the speed can reach 1000 events/sec, far exceeding other brands of microfluidic sorting devices.

5. It only takes 5 minutes from startup to sample completion, eliminating long waiting times.

6. Using disposable microfluidic chips, there is no need for cleaning and maintenance, and there is no possibility of cross contamination.

7. Accurate fluid control, optional culture medium or oil as sheath fluid, enables a wide range of sample detection.

8. The technology can obtain SSC parameters from microfluidic chips.

9. Microfluidic chips of different specifications can provide a wide range of sample size options, with a maximum analyzed sample diameter of up to 125 μ m.

10. Fast and accurate single-cell plating, with a single cell plating accuracy of over 90%.

11. Built in HEPA filter keeps the air inside the instrument clean, and the instrument can be placed in a customized anaerobic glove box.

On-chip Droplet SelectorWidely applicable research areas:

Environmental microbiology field

Discovering new microorganisms in the environment

Enzyme engineering field

Quickly establish high-yield bacterial strainswait

In the field of drug development

Screening antibody producing cellswait

Application case 1: Single droplet plating of encapsulated rhizobium droplets

About 5% of all droplets encapsulate rhizobia. Droplets undergoing rhizobium proliferation can be dispensed into a 96 well plate using an On chip Droplet selector. After cultivation in a well plate, 32 out of 45 wells were found to have increased proliferation of rhizobia.

Application Case 2: Screening of Enzyme Producing Microorganisms in Soil

Encapsulate the reaction substrates of microorganisms and enzymes obtained from soil together in droplets and cultivate them. In the experimental results, various forms of microbial proliferation and enzyme activity were observed. The On chip Droplet selector can perform high-speed sorting of droplets based on their different enzyme activities, aiding in the discovery of new microbial species.

Application Cases3Detecting microorganisms that proliferate in droplets

FNAP-sort (Fluorescent Nucleic Acid Probein dropletsforsorting bacteria)It is a reaction system that can be used to detect microbial proliferation within droplets.

In the example of droplet culture of Escherichia coli, Bacillus subtilis, Streptomyces aureus, and soybean rhizobia, the proliferation ability of microorganisms is positively correlated with fluorescence intensity, making it easy to detect proliferation ability based on this. Through this system, microorganisms or mutant strains in the environment can be screened based on their proliferative activity.