Low temperature liquid nitrogen grinder

In molecular biology experiments, animal muscle tissue, connective tissue, bone tissue, hair tissue, and plant roots, stems, leaves, seeds, etc. need to be ground to study their composition, genes, proteins, structure, and function.

In order to prevent various tissue components from being easily damaged or degraded during the grinding process, and to increase the hardness and brittleness of the tissue, making it easier to grind, especially for the grinding, separation, and extraction of ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). It is necessary to first rapidly freeze the biological material in liquid nitrogen to terminate all biological reactions inside and outside the cell. At the same time, the biological material cells will freeze hard and become brittle. This grinding can achieve good cell breaking effect, grinding the cells into powder and releasing the substances inside.

Plant tissues: roots, stems, leaves, flowers, fruits, seeds, etc;

Animal tissues: brain, heart, lungs, stomach, liver, thymus, kidneys, intestines, lymph nodes, muscles, bones, etc;

Wood, seeds, dense soil, and sandy soil, etc;

Fungi and bacteria: yeast, Escherichia coli, spores, etc;

Ancient dry samples, rust, etc;

◇ Whole insects, algae, coral lotion, etc.

1. The liquid nitrogen grinder divides the tissue sample into suitable centrifuge tubes.

2. Add an appropriate amount of cracking medium into the centrifuge tube.

3. Tighten the lid of the centrifuge and insert it into the freezer adapter.

4. Use an adapter fixture to place the frozen adapter as a whole in liquid nitrogen for 3-15 minutes.

5. Use the adapter fixture again to place and remove the frozen adapter as a whole, place it on the Gd16plus grinder, and tighten the fixed handle.

6. Set the grinding speed and time, and start the Gd16plus grinder.

7.Low temperature liquid nitrogen grinderAfter stopping, remove the freezing adapter and take out the centrifuge tube.

1. Liquid nitrogen has a relatively low temperature at atmospheric pressure, so extra attention should be paid when using liquid nitrogen to avoid direct contact with the skin.

2. The liquid nitrogen grinder needs to place the freezing adapter in liquid nitrogen and remove it from the liquid nitrogen using an adapter fixture, and wear antifreeze gloves.