Lipopolysaccharide derived from Escherichia coli 055: B5 (LPS)

NegotiableUpdate on 05/06

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Overview

Lipopolysaccharide is derived from Escherichia coli 055: B5 (LPS), abbreviated as LPS. It is a component of the cell wall of Gram negative bacteria (G -), located at the outermost layer of the cell wall and exposed to the cell surface of non enveloped bacteria. It helps maintain the integrity of the cell outer membrane and protects bacteria from damage caused by bile salts and lipid antibiotics. LPS is composed of lipid A, core polysaccharides, and O-polysaccharide side chains. Lipid class A is the main component of bacterial endotoxins, determining their toxicity. The high variation of O-polysaccharide side chains among different bacteria determines the specificity of bacterial serotypes

Product Details

Lipopolysaccharide derived from Escherichia coli 055: B5 (LPS)

Product Description

Lipopolysaccharides, also known as LPS, are a component of the cell wall of Gram negative bacteria (G -). They are located on the outermost layer of the cell wall and are exposed to the cell surface of non enveloped bacteria. They help maintain the integrity of the cell outer membrane and protect bacteria from damage caused by bile salts and lipid antibiotics. LPS is composed of lipid A, core polysaccharides, and O-polysaccharide side chains. Lipids A are the main components of bacterial endotoxins, determining their strength. The high degree of variation of O-polysaccharide side chains among different xi bacteria determines the specificity of xi bacterial serotypes.

LPS can cause a cascade reaction of immune stimulation and toxic pathophysiological activities in the body, including the release of endotoxins leading to septic shock and peripheral vascular collapse. Meningitis is often diagnosed clinically by detecting the presence of LPS. It is precisely the close relationship between LPS and the immune function of the body that life science research often extracts LPS for related studies, such as elucidating the structure, metabolism, immunology, physiology, toxicity, and biosynthetic pathways of LPS; Inducing the synthesis and secretion of growth promoting factors such as interleukin; Animal models for inducing disease research, such as inflammatory response and acute lung injury. LPS has high immunity and strongly activates immune cells carrying CD14/TLR4/MD2 receptor complexes. FITC, TRITC, or TNP labeled LPS are commonly used to study the immune response of T cell-independent B cells to it.

This product is derived from Escherichia coli serotype 055: B5, extracted with benphenol, and the strain is CDC 1644-70. The endotoxin level is ≥ 500000 EU/mg. After analysis, 1ng is equivalent to 5EU (horseshoe crab reagent method) and 10EU (colorimetric method). This product can be used to stimulate human peritoneal macrophages (1ng/ml) and horse peritoneal macrophages (1-100ng/ml).Our company also providesSource fromEscherichia coli serotypeO111:B4Lipopolysaccharide（LPS）(CAT:#FSY0328).

The differences between two lipopolysaccharides derived from Escherichia coli are as follows:

Item Number	CAT:#FS0203	CAT:#FSY0328
name	Lipopolysaccharide derived from Escherichia coliO55:B5	Lipopolysaccharide derived from Escherichia coliO111:B4
serotype	O55:B5	O111:B4
Main applications	In vitro cell activation; Used for endotoxinsLALtesting; As a vaccine adjuvant to enhance immunogenicity; Used for the study of gan staining and inflammatory response mechanisms; Perhaps more suitable for in vitro cell activation and inflammation research.	Stimulate the inflammatory response of pig liver tissue in vivo and piglet ileum tissue in vitro; Stimulating natural killers outside the body(NK)Cells and liverBCells; Inducing arthritis in the standard rabbit model; Perhaps more suitable for researchBCell activation andNOSinduce.
similarities	Both can be achieved by interacting with the surface of host cellsTollReceptor like receptor4（TLR4）Combining to activate innate immune system cells and trigger inflammatory responses; Both have immunogenicity and can stimulate immune cells to produce inflammatory factors, which are used to simulate the process of bacterial infection; Both are widely used in biomedical research, especially in the study of inflammation and immune response mechanisms.

Product Features

AppearanceWhite to yellow brown powder;

Purification methodBen phenol extraction;

Impurities:<3% protein (determined by Lowry method)；

solubilityDissolve in water (5mg/ml) or cell culture medium (1mg/ml)

Storage and transportation methods:2-8 ℃, valid for at least 5 years, transported in ice packs

Usage

1. Preparation of LPS storage solution (related to cell culture): Resuspend 1mg of LPS in 1ml of sterile equilibrium solution or cell culture medium, gently vortex and shake until the powder is completely dissolved, resulting in 1mg/ml storage solution. This storage solution can be further diluted with sterile equilibration medium or cell culture medium to the desired working concentration.

2. Preservation of LPS storage solution: This storage solution (1mg/ml) can be stored at 2-8 ° C and remains stable for about a month; It can also be divided into single doses and stored at -20 ℃ for up to 2 years of stability. Avoid repeated freezing and thawing.

[Attention]:

1. LPS storage solution should be stored in a silanization container, as LPS can adsorb onto plastic or certain glassware, especially when the concentration is less than 0.1mg/ml. But when the concentration of LPS is greater than 1mg/ml, the above-mentioned adsorption loss can be ignored.

If using glassware, vortex oscillation of LPS storage solution should be carried out for at least 30 minutes to fully dissolve the adsorbed solute.

Precautions

1) Due to the fact that LPS is a micelle forming molecule in any solvent, LPS solutions dissolved in water and phosphate are slightly turbid to turbid. Organic solvents will not make the LPS solution clearer. The dissolution of Jia Chun produces a chaotic suspension with suspended particles. However, water dissolution produces a homogeneous turbid liquid.

2) For your safety and health, please wear lab coats and disposable gloves when operating.

Lipopolysaccharide derived from Escherichia coli 055: B5 (LPS)