J2 Anti dsRNA Monoclonal Antibody Proxy

J2 monoclonal antibody (mAb), mouse, IgG2a, kappa chain

J2 monoclonal antibody (mAb), mouse, IgG2a, kappa chain

J2 monoclonal antibody (mAb J2) is a mouse derived IgG2a antibody that targets double RNA (dsRNA). Its recognition ability plays an important role in virus detection and research. The J2 antibody was initially developed for the detection of plant viruses, but with further research, its application scope rapidly expanded. In 2006, Weber et al. confirmed in a study that J2 antibodies can detect a large amount of dsRNA produced by all positive stranded RNA viruses in infected cells. This discovery has made J2 antibody the "gold standard" for detecting dsRNA and has driven its widespread application in related fields, with over 200 related literature published so far.

J2 Anti dsRNA Monoclonal Antibody ProxyFeatures and Applications

J2 antibody can recognize dsRNAs with a length greater than or equal to 40 bp, and its recognition process does not depend on the sequence or nucleotide composition of the antigen. After research, it was found that J2 antibody can recognize various naturally occurring dsRNAs, including hepatitis C virus, dengue virus, rhinovirus, chikungunya virus, rabies virus, poliovirus, swine fever virus, and sparrow straw mosaic virus. This makes J2 antibody a tool in virological research. This antibody is suitable for various experimental techniques, including enzyme-linked immunosorbent assay (ELISA), dsRNA immunoblotting, immunoaffinity chromatography, and immunohistochemistry in certain systems. It should be noted that before dsRNA immunoblotting, nucleic acid separation must be performed by polyacrylamide gel electrophoresis, because the sensitivity after transfer from agarose gel will be significantly reduced. In addition, J2 antibodies are not suitable for clinical use and are limited to in vitro experiments.

J2 monoclonal antibody storage and stability

Before use, J2 monoclonal antibodies are typically provided in freeze-dried form. After reconstitution, it is recommended to pack and store it at -20 ° C or -70 ° C to ensure its activity and stability.

Background and literature support of J2 monoclonal antibody research

The development of J2 monoclonal antibodies is based on a deep understanding of the structure of double stranded RNA. As early as 1991, Sch ö nborn et al. published research demonstrating how monoclonal antibodies targeting double stranded RNA could serve as RNA structure probes in nucleic acid extracts. In the following years, Lukacs et al. further explored the application of this antibody in the detection of plant virus infections and proposed a method for distinguishing coexisting viruses using monoclonal antibodies. These studies have laid the foundation for the widespread application of J2 antibodies. Currently, SCICONS is a manufacturer of mouse monoclonal antibodies that hybridize with double stranded RNA molecules, including K1, K2, and J2 monoclonal antibodies. SCICONS is headquartered in Hungary and its customers are located in 35 countries worldwide, with over half of the 50 states in the United States using SCICONS monoclonal antibodies. The company is committed to providing high-quality research tools for international research institutions and has gained recognition from numerous scholars.

J2 Anti dsRNA Monoclonal Antibody Proxyorder

References
Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000.

Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272.

Lukacs, N. (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein amd W. Nellen (eds), pp. 281-295. IRL Press, Oxford.

SCICONS Company Introduction

SCICONS Hungary is a global provider of mouse monoclonal antibodies that can hybridize with double stranded RNA molecules, including K1 monoclonal antibodies, K2 monoclonal antibodies, and J2 monoclonal antibodies.
SCICONS, also known as English&Scientific Consulting Kft, is headquartered in Szir á k, Hungary. SCICONS has clients in 35 countries worldwide, and over half of the 50 states in the United States use SCICONS monoclonal antibodies. SCICONS' customer base includes scholars from many international research institutions, who have published over 200 scientific articles. See link: documented by over 200 scientific articles.

NORDIC MUBIO Company Introduction

Nordic Miubio develops and manufactures high-quality antibody reagents for research and development. All products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies against cell adhesion proteins, nuclear proteins, and cytoskeletal proteins.

Nordic MUbio is one of the antibody companies that researches and develops antibodies for zebrafish. Currently, we have over 50 validated antibodies that can be used for immunofluorescence in zebrafish.

In the collaborative development project, Nordic MUbio is researching, testing, and validating antibodies and antigens for diagnostic applications in the field of lung cancer and other cancers.