Although primary human renal epithelial cells can most accurately reflect the physiological state of renal epithelial cells in the human body, on the one hand, human kidney tissue is very precious, and primary isolation and cultivation of human renal epithelial cells have a long cell cycle and require high technical experience. On the other hand, the growth of these primary cells is slow and cannot be passaged in vitro, which restricts their widespread application in the laboratory. The research team of Xinrun Biology has years of experience in primary cell isolation, culture, and cell immortalization services, and has successfully established immortalized human renal epithelial cells.
Renal tubular epithelial cells play an important role in the functioning of the kidney. Renal tubular epithelial cells can produce inflammatory mediators including cytokines and chemokines, and actively participate in the acute inflammatory process by producing IL-8 to influence and guide the differentiation of white blood cells. During the inflammatory process of kidney transplantation or crescentic glomerulonephritis, renal tubular epithelial cells express IL-2 α receptors and class II MHC antigens, indicating their involvement in the pathogenesis of renal immune system damage. The renal epithelial cell line is derived from primary renal tubular epithelial cells.
The outer layer of the glomerulus is the epithelial cell layer, also known as podocytes. Its irregular protrusions are called podocytes, with many narrow gaps between them. After blood is filtered through a filter membrane, the filtrate enters the renal glomerulus. Under normal circumstances, the vast majority of proteins in the blood cannot be filtered and remain in the bloodstream, only small molecule substances such as urea, glucose, electrolytes, and certain small molecule proteins can be filtered.
The products produced by our companyHuman renal epithelial cell linePrepared by enzymatic hydrolysis and gene transfection, the total number of cells is approximately 1 × 106/The T25 square bottle has a cell purity of over 90% and does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, fungi, etc.
Culture medium information:
We recommend using the product developed by Xinrun BiotechnologyHuman renal epithelial cellsbeculture medium(Product Code: IH1012-5) as an in vitro cultureHuman renal epithelial cell lineThe cultivation medium.
