Histone methylation, phosphorylation, acetylation detection experiment

Histone methylation, phosphorylation, acetylation detection experiment

Histone methylation, phosphorylation, and acylation detection:

This test includesMethylated histoneH3K4、 Methylated histone H3K9. Methyled histone H3K27. Histone H3 phosphorylation (Ser10) detection, histone H3 phosphorylation (Ser28) detection, histone H3 acetylation detection, histone H4 acetylation detection, etc.

This method is based on specific antibody detection of histone components, and the content of each component is determined by colorimetric quantification.

Experimental period: Completed within 10-12 working days, with repeat testing discounts.

Customer precautions

1. Antibodies:

Consult with Yanjin Biotechnology in advance to clarify the antibody species and whether the antibody is available for use; If there is no agreement between the two parties, the customer can purchase and provide it themselves or entrust it to our company for purchasing.

2. Specimen collection and preservation:

① Organizational samples: Fresh tissue should be stored in liquid nitrogen or at -70 degrees Celsius, with a minimum of 100mg of tissue (approximately the size of a mung bean);

② Cultivate cells: Take no less than 2000000 cells through cell counting and transfer them to an EP tube. Add 0.5ml of physiological saline or protein protectant, mix well, and store in a refrigerator (-70 ° C to avoid repeated freezing and thawing);

③ Blood cell specimen: Store the blood sample in an anticoagulant tube or separate the cells in lymphocyte isolation solution and add 0.5ml of physiological saline or protein protectant for preservation (can be stored for a long time at -70 ° C to avoid repeated freezing and thawing)

④ Serum or cell culture medium specimens: After centrifugation to remove impurities, take the supernatant and transfer it into an EP tube for storage (above 4 ° C for 15-20 days, -70 ° C for long-term storage to avoid repeated freezing and thawing)

3. Sample transportation:

You can choose any of the following ways to send specimens

① Organizational samples and cell samples are transported by dry ice or added with protein protectants and then transported in ice packs;

② Cell samples can also be sent directly to the culture bottle by express delivery (sealed to ensure no contamination, no leakage of culture medium, cells should not grow too full, about 50%, filled with culture medium);

③ Serum or supernatant can be directly sent in ice packs (sealed to ensure that the liquid does not leak, depending on the distance of the journey, it can take 2-3 days to arrive).

Experimental outsourcing service: