Enzyme activity detection experiment

Enzyme activity detection

Service Introduction

Enzyme activity detection (kinase, esterase, phosphatase, dehydrogenase, synthase) is the measurement of enzymes (kinase, esterase, phosphatase, dehydrogenase, synthase) in human serum or plasma

The catalytic activity of enzymes, esterases, phosphatases, dehydrogenases, and synthases. The commonly used methods are endpoint method and kinetic method.

Generally, commercial reagent kits are used for measurement.

2、 Experimental principle

The experimental principles used for different reagent kits are different, such as lipase assay kit (enzyme colorimetric method): indirectly indicating the activity of the enzyme by detecting the absorbance of the sample; Kinase assay kit: Based on the kinase's ability to catalyze the phosphorylation of specific substrates, 32y ATP is added externally. After separating the reaction products, the radioactivity is measured, and the activity of the sample enzyme is calculated from the standard curve.

3、 Experimental process

1. Sample preparation;

2. Testing with reagent kits and biochemical analyzers;

3. Data organization and analysis.

4、 Service Description

1. The customer provides:Serum and plasma.

2 companies provide:Basic experimental steps and data analysis results.

3 Experimental cycles:

About 1-2 weeks, depending on the specific experimental content.

5、 Quality Commitment

Provide clear images, reliable detection data, and analysis results.

Experimental outsourcing service: