High sensitivity improved Kato's parasite egg detection slide box

Instruction Manual for Parasite Egg Detection Preparation

（Improved Kato's method）

Introduction:The improved Kato's method is the responsibility of the World Health Organization(WHO)The recommended method for detecting parasite eggs can simultaneously detect roundworm eggs, hookworm eggs, liver fluke eggs, and so on. This detection kit is used as an auxiliary tool for microscopic detection of parasite eggs in feces, which can improve the effectiveness of parasite detection and quantitatively analyze the degree of infection.

Composition of detection package:

1Composite dye solution1bottle 50milliliter/bottle

2Quantitative board 100piece

3Scraper blade 100piece

4Nylon mesh 100Zhang

5Glass paper 200Zhang

6, glass slides 100piece

Operation steps:

1、Place the nylon strip on the fecal sample to be tested, and gently scrape it with a plastic scraper to allow fine fecal residue to pass through the holes of the nylon strip，Take filtered feces.
2、Place the quantitative plate in the middle of the glass slide, and then fill the fine fecal residue on the scraper into the circular hole of the quantitative plate, filling the entire hole and smoothing it out. Carefully remove the quantitative plate, leaving the fecal sample on the glass slide.

3The取1Soak the soaked hydrophilic glass paper, shake off excess transparent liquid, cover it on the fecal sample, and gently press it with a thick glass slide to evenly spread the fecal matter under the glass paper.
4、Place the prepared Kato tablets in the specimen box (lined with absorbent paper).

5After numberingKeep at room temperature25℃Humidity75%Time Schedule1Hour or30~36℃After about half an hour in the incubator, conduct a microscopic examination.

microscopic examinationandResult judgment:

1、Microscopic examination: Place the transparent Kato film on the stage of an optical microscope and examine it under a low magnification mirror（10×）Inspect under the microscope. Microscopic examination carefully examines each field of view and pays special attention to the identification of unfertilized roundworm eggs. Count the entire insect eggs (counting principle: count up but not down, count left but not right).

2、Egg count of Kato tablets:The number of insect eggs detected by microscopic examination per piece24Multiple (one stool, one test)，That is, the number of eggs per gram of feces（EPG）.

calculate3The number of schistosomiasis eggs in Zhang Kato's slice isATheBTheC, number of eggs per gram of feces(EPG)For:(A+B+C) ×8(One stool and three tests).

Notes:

1Each fecal sample requires three smears (one stool and three tests to improve detection rate)

2Cut glass paper should be twisted into pieces and placed in transparent liquid. Avoid putting it in a whole stack to prevent the inside from being soaked and affecting the transparency effect; The soaking time cannot be less than24Hour.

3The maximum transparent time for thin shell eggs, such as hookworm eggs, cannot exceed2Hour by hour, to avoid missed detections due to excessive transparency.

4、When the feces are hard, soak them in a little water or clear liquid for a moment, and then scrape them off.

5、The quantitative plate must be placed in the middle of the glass slide. If the quantitative plate is biased to one side, the fecal film at the edge of the slide cannot enter the field of microscopic inspection after slicing, resulting in missed detection.
6.When making specimens, excessive drying should be avoided to prevent deformation or rupture of the insect eggs, which may affect their identification.
7.The speed at which fecal residue becomes transparent depends on temperature, humidity, and the thickness of the fecal film. Generally, it can be left at room temperature, but in winter, it needs to be placed in a temperature chamber to accelerate transparency. If the fecal membrane is too thick, it is difficult to find the eggs.

This testing production package is available forroom temperaturepreservation,24Valid within one month.

Specifications 100personcopy/package For research purposes only, not for clinical diagnosis!Manufacturer: Yikangda Technology (Guangzhou) Co., Ltd