High sensitivity camera

1High sensitivity cameraProduct Introduction

DhyanA400BSI has received great attention since its launch, and its product quality has been fully recognized. But we have never stopped moving forward. With the collective efforts of our R&D personnel, we have now completed the comprehensive upgrade of 400BSI from V1.0 to V2.0.

400BSI (V2.0) has achieved a core breakthrough in transmission speed compared to V1.0, and can achieve full resolution 74fps@Cameralink and 40fps@USB3.0 High speed data transmission. In signal processing, in order to meet the precise quantitative requirements of scientific imaging such as single-molecule and super-resolution, 400BSI (V2.0) has calibrated DSNU and PRNU, reducing the differences between each pixel and fixed pattern noise to a very low level, thereby achieving higher precision quantitative imaging and providing guarantees for obtaining more accurate application data.

In addition, 400BSI (V2.0) retains all the essence of V1.0, including the latest back illuminated sCMOS chip, ultra-high quantum efficiency with 95% QE, pixel size of 6.5 μ m x 6.5 μ m, ultra-low readout noise of 1.1e - @ CMS, and other excellent qualities.

Whether it's capturing stunning scientific images or obtaining precise quantitative data, DhyanA400BSI (V2.0) can handle it with ease!

IIHigh sensitivity cameraProduct advantages

1. Pixel correction, DSNU/PRNU optimization, more accurate quantitative analysis

To improve the overall performance of the camera, DhyanA400BSIV2.0 has optimized the correction of DSNU (dark signal non-uniformity) and PRNU (light response non-uniformity). The optimized DSNU value has been reduced from 0.3e - to 0.2e -, and the PRNU value has been reduced from 1.6% to 0.3%. As a result, the camera has more precise quantitative capabilities and is more suitable for quantitative analysis applications.

2. Low temperature dark current control at -15 ℃ to further reduce the impact of noise on imaging

The Dhyana 400BSI V2.0 can achieve a cooling temperature of -15 ℃ at 20 room temperature. Compared with the readout noise of 1.2e - and the DSNU of 0.2e -, the dark current noise corresponding to 100 milliseconds is less than 0.02e -, which is almost negligible for sCMOS imaging applications.

3. Wide spectral response range, high sensitivity unattainable

When all noise indicators and good levels are comparable, the quantum efficiency advantage of Dhyana 400BSIV2.0 is highlighted, which is a significant breakthrough for scientific applications. Not only has the sensitivity been greatly improved in the visible light region, but the improvement in ultraviolet short wave and near-infrared long wave is also worth paying attention to.

4 74fps@CameraLink , 40fps@USB3.0 Faster data capture

In addition to its signal-to-noise ratio advantage, Dhyana 400BSI V2.0 has also achieved a core breakthrough in transmission speed. On the one hand, it has added a CameraLink interface to meet the demand for higher frame rates in scientific imaging. On the other hand, it has upgraded and improved the internal hardware circuit, ultimately achieving 4.2 million full resolution 74fps@CameraLink The maximum transmission rate of the chip, and 40fps@USB3.0 The highest data transmission rate of the interface.

3、 Third party applications

In addition to its own SDK and demo, Dhyana 400BSI V2.0 has greatly expanded its support for third-party applications, including Micromanager

Labview, Matlab, and other tools can provide you with more application support and assistance.

4、 Application Case Reference

Single-molecule localization

The high signal-to-noise ratio of the camera can effectively improve the brightness of single-molecule fluorescence emission. The statistical results in the right figure show that the positioning accuracy of 400BSI is twice that of the comparative camera. Data source: Wuhan National Laboratory of Optoelectronics, Huazhong University of Science and Technology.

Super-resolution imaging

The smaller the full width at half maximum (FWHM), the higher the resolution. In STORM cell super-resolution imaging, 400BSI achieved a resolution of 40 nanometers, while the third-generation 82% QE sCMOS could only achieve a resolution of 47 nanometers. 400BSI advanced the resolution of STORM ultra-high resolution microscopy by 7 nanometers!

Neuron fluorescence imaging

As the exposure time increases, the luminescent fluorescent substance will produce phototoxicity to cells. Compared with other cameras, the imaging exposure time of 400BSI is shorter, which can better

Protect cell samples from light damage.

TIRF Wide Field Imaging

In total internal reflection fluorescence (TIRF) applications, the light intensity is very weak, but the ultra-high signal-to-noise ratio of 400BSI can effectively reduce exposure time and achieve faster wide field imaging speed.

SIM Cytoskeleton Imaging

SIM imaging requires the camera to obtain as clear an image as possible at low exposure times, and under the same shooting conditions, 400BSI has a significant signal-to-noise ratio advantage, thus achieving better imaging quality than other cameras.