Gibco South American fetal bovine serum A5256701 price

Gibco South American fetal bovine serum A5256701 price

In basic cell culture, tumor research, and biopharmaceutical development, fetal bovine serum is used asThe quality and stability of natural culture media directly affect the reproducibility of cell growth status and experimental results. Gibco South American fetal bovine serum A5256701 as a subsidiary of Thermo Fisher ScientificThe Brazilian sourced premium fetal bovine serum approved by the General Administration of Customs of China for entry into China meets the premium standards of endotoxin ≤ 10EU/mL and hemoglobin ≤ 25mg/dL, providing researchers with a new option that balances quality and cost. This product has been sold globally for over 30 years, to multiple countries and regions, and has supported the publication of over 8000 SCI papers. With a strict quality control system and compliant import qualifications, it is becoming a reliable alternative to traditional South American blood derived serum for conventional cell culture.

Choosing Gibco South American fetal bovine serum A5256701 means choosing a globally validated and cost-effective nutritional support for your daily cell culture work.

1、 Brazil sourced fetal bovine serum from Thermo Fisher's compliant entry into China

The launch of Gibco South American fetal bovine serum A5256701 is a milestone. In April 2024, the General Administration of Customs of China officially allowed the export of Brazilian fetal bovine serum to China; In August of the same year, the product becameBrazilian fetal bovine serum, approved by the General Administration of Customs of China, entered China in compliance with Shanghai Pudong International Airport regulations.

Each bottle of Gibco South American fetal bovine serum A5256701 is equipped with a complete set of compliance documents:

Hygiene Certificate for Brazilian Beef Blood Products Exported to China

Entry Animal and Plant Quarantine License

Customs declaration form and quality inspection report (CoA)

This complete qualification system ensures the traceability of product sources, effectively avoiding legal and quality risks of informal channel products, and providing cost-effective alternatives to Australian and New Zealand serum for scientific research and industrial users.

Strict quality control system

Gibco South American fetal bovine serum A5256701 undergoes nearly 30 quality tests, following 9CFR and EMA standards, and up to 11 virus screenings,Ensure the safety of products, operators, and final products. Its core quality indicators include:

Parameter category specific specifications

Serum grade excellent (Value)

Bloodborne Brazil (product label indicating blood source location)

Endotoxin level ≤ 10EU/mL

Hemoglobin level ≤ 25mg/dL

Sterilization method: 0.1 μ m triple filtration

Packaging specification: 500mL bottle

Storage temperature -5 ℃ to -20 ℃

Valid for 5 years

The product is produced in FDA registered factories that comply with cGMP requirements, certified according to ISO13485 standards, and all batches of serum undergo strict testing to ensure their safety and effectiveness. The performance differences between batches are small, effectively improving the reproducibility of experiments.

2、 Stable global supply guarantee

Brazil is an important supplier of fetal bovine serum worldwide, with a land area of approximately 8.5 million square kilometers, almost half of South America, and possessingCommercial cattle herd, approximately 207 million heads. The production of fetal bovine serum accounts for nearly 60% in South America and 23% globally, second only to the United States, followed closely by Australia and New Zealand. This production capacity foundation, combined with Gibco's over 60 years of professional quality verification, provides stable supply guarantee for scientific research users and effectively alleviates the tight supply of high-quality fetal bovine serum in recent years.

3、 Applicable cell types and application scenarios

The main choice for conventional cell culture

Gibco South American fetal bovine serum A5256701 is suitable for conventional cell culture and provides reliable nutritional support for the following cell types:

Passage cell lines: HEK293, HeLa, CHO, 293T, A549, etc

Tumor cell lines: MCF-7, BT-474, B16F10, SKOV3, Raji, etc

Partial primary cells: human PBMCCD8+T cells, mouse spleen CD8+T cells, rat primary cardiomyocytes, etc

User feedback shows that after using this serum, the cell state is good, the recovery survival rate is high, the adhesion effect is ideal, and the proliferation rate is stable.

Validation of over 8000 global literature

Before officially entering the Chinese market, Gibco's South American fetal bovine serum A5256701 had been sold globally for over 30 years, to multiple countries and regions, and was widely recognized by researchers and biopharmaceutical companies worldwide, with over 8000 published papers.

Among them, there are research results published in high-level journals such as Nature Metabolism and Nature Communications. For example, in the study published in Nature Metabolism in 2023 titled "Glutarateregulars Tcell Metabolizamanti tumourimmunity," this serum was used for both immune cell and tumor cell cultures.

Two special processing models: meet different experimental needs

In addition to the standard model A5256701, the Gibco South American fetal bovine serum series also offers specially processed versions to meet different experimental scenarios:

Product number: Thermal inactivation Gamma irradiation applicable scenarios

A5256701 No No Conventional cell culture

Is A5256501 for biopharmaceutical or vaccine research and development

A5256901 is an immunological experiment involving sensitive cells

Gamma irradiation treatment can effectively inactivate potential viruses and mycoplasma, making it suitable for applications with high biosafety requirements such as biopharmaceuticals and vaccine development. Heat inactivation can remove complement activity and avoid complement dependent cytotoxicity interference, making it suitable for immunological experiments, ADCC/ADC detection, and other studies that require exclusion of complement interference.

4、 Usage and Storage Guide

The correct storage and usage methods are crucial for maintaining the quality of Gibco South American fetal bovine serum A5256701.

Storage conditions

Long term storage: Freeze stored at -5 ℃ to -20 ℃, with a shelf life of 5 years

Short term storage: After thawing at 4 ℃, the short-term storage should not exceed one month and must be strictly sealed to prevent contamination

Avoid repeated freeze-thaw cycles: It is recommended to pack according to a single dose and reserve 10% expansion space to prevent container rupture

Thawing method

Recommend using gradient thawing method:

Transfer the serum from -20 ℃ to 2-8 ℃ and thaw overnight in the refrigerator

Gently shake every 2 hours during the thawing process to ensure even temperature and composition

Do not thaw directly in a 37 ℃ water bath from -20 ℃ to avoid excessive temperature changes that may cause protein aggregation and precipitation

Suggestions for packaging and preservation

Packing volume: 40-45mL per tube is recommended (the volume will increase by about 10% when serum freezes)

Mix well before packaging: Ensure that the components of each tube are consistent to avoid quality differences caused by uneven distribution of sediment

Marking: Mark the packaging time and batch number on the pipe wall for easy traceability

Precipitation treatment

After thawing, serum may form flocculent precipitates, usually caused by lipoprotein denaturation or fibrin precipitation, which will not affect its performance. If removal is required, the serum can be centrifuged at 400 × g for 5 minutes, and the supernatant can be used. It is not recommended to filter (flocculent substances may block the filter membrane).

Thermal inactivation judgment

Unless immunological experiments require complement inactivation, conventional cell culture does not require heat inactivation. Heat inactivation (56 ° C, 30 minutes) can inactivate complement, but it can also increase sediment and affect serum quality. If thermal inactivation is required, shake it regularly and evenly in the water bath to avoid gel of serum caused by excessive temperature or time.

5、 Selection reference and procurement suggestions

When selecting Gibco South American fetal bovine serum A5256701, the following factors can be considered:

Recommendation of decision-making dimensions and core considerations for selection

Cell types: Conventional passaged cells, tumor cells A5256701 with superior quality to meet the needs of most conventional cells

Aseptic requirements: Conventional cultivation standard type; Higher requirements for the biological safety level of A5256501 (Gamma irradiation type)

The experiment requires conventional cultivation of standard types; Special Requirements for the Experimental Design of Immunology Experiment A5256901 (Heat Inactivated Type)

Usage Requirement: Regular Usage: 500mL Bottled to Avoid Repeated Freezing and Thawing Affects Quality

Budget conditions pursue cost-effectiveness. Brazilian source A5256701 is more competitive in serum price compared to Australian source

summary

In conclusion,Gibco South American fetal bovine serum A5256701With its compliant import qualifications, strict 30 quality tests, low endotoxin levels of ≤ 10EU/mL, and verification of over 8000 global literature, it has become a reliable choice for conventional cell culture. Whether it is the daily maintenance of passaged cell lines or the cultivation optimization of some primary cells, this product can provide more competitive cost-effectiveness while ensuring cell health. Choosing the appropriate model based on your cell type and experimental requirements, and following the standardized packaging and thawing process, will help improve the success rate and data reliability of cell culture work.

Gibco South American fetal bovine serum A5256701 pricePlease consult us for the latest purchasing price. Hangzhou Qiannuo Biological Warehouse has a large reserve of frozen serum for direct shipment nationwide.