Fully automatic exosome concentration and purification instrument

This instrument adopts a pulsating tangential flow multi-stage stable pressure filtration method to achieve fully automatic extraction, concentration, purification, and liquid exchange processes of extracellular vesicles from samples; Widely used in scientific research on extracellular vesicles from cell supernatants, animal body fluids, and plant extracts;

1. Main functional parameters:

(1) Sample processing volume: includes two sample processing modes: 10 μ l-500ml and 10000ml. Different types of filtering units can be used to achieve different sample extraction processes;

(2) Supporting sample types: various body fluids, stem cell supernatants, plant plasma extracts;

(3) Sample position supports test tube types: storage tank types between 15ml-450ml;

(4) Sample addition method: fully automatic mode, which includes the full process automation of instrument automatic sample loading, concentration, purification, liquid exchange, and automatic collection;

(5) Reagent management: The product is equipped with injection grade physiological saline and sterile PBS physiological solution, which can clean and replace extracellular vesicles in real time;

(6) Consumables type: The extracellular vesicle filtration unit includes multiple sub models, as shown in the table below;

(7) Single maximum sample volume: The product processing sample volume includes two modes, one is 10-500 milliliters mode and the other is 10 liters mode, which can be selected according to the filtering unit;

(8) Extracellular vesicle purification and enrichment process: The product includes two-stage purification and concentration functions, and the combination of three functions can achieve synchronous purification and concentration of extracellular vesicles;

(9) Purification time: The extraction time for a 200ml sample is less than 10 minutes;

(10) Processing speed: The extraction rate of the product is 1-3 hours/10 liters;

(11) Extracellular vesicle recovery volume: capable of setting the volume of extracellular vesicle concentrate;

(12) Extracellular vesicle subtype separation: Four different pore sizes of membrane packs are 0.45um, 0.22um, 0.1um, and 0.04um, respectively. The four different pore sizes of membrane packs used by the equipment for filtration are stored in the first filtration tank (particle size in the first filtration tank is 200-450nm), the second filtration tank (particle size in the second filtration tank is 100-200nm), and the third filtration tank (particle size in the third filtration tank is 30-100nm), which can be selected according to user needs.

(13) Temperature control: The product is equipped with 7 weighing sensors, 5 pressure sensors, and 2 temperature sensors. It adopts a fuzzy PID control strategy, which can intelligently identify the filtering unit and automatically achieve concentration and purification of different volumes of extracellular vesicles, achieving full process temperature control;

(14) Transparent visualization warehouse door: equipped with multiple heavy sensors and adopting fuzzy PID control strategy, it can intelligently identify filtering units, achieve full process visualization of the processing, and automatically achieve concentration and purification of different volumes of extracellular vesicles;

(15) Purification process: The product has multiple sensors, and the control software is equipped with multiple operating modes. Through combination, dozens of concentration and purification modes can be achieved, which can optimize the concentration and purification of extracellular vesicles;

(16) Review list data storage: Equipped with a micro built-in industrial computer, the historical data storage space is 300G, and the data can be stored for 10 years;

(17) Screen display: The 11.4-inch display screen can achieve scanning and code input functions, set initial sample parameters, real-time display of historical data, storage of historical data, parameter alarm and other functions. Each batch has a fixed code for easy query and filing;

(18) Pollution prevention and control: Equipped with two ozone free ultraviolet lamps, the sterilization time can be freely set, and the system defaults to a sterilization time of 30 minutes;

(19) Maintenance: Equipped with injection grade physiological saline and sterile PBS, the software is equipped with a cleaning process, which can achieve functions such as leakage detection, filtration unit cleaning, and automatic recovery;

(20) Removal rate of impurities: greater than 99%;

(21) Extracellular vesicle recovery rate: greater than 90%;

(22) Various sample machine volumes: The machine volumes and types of samples are already covered in the product functions and extraction types of 10 company, which can meet the requirements;

(23) Operation interface: Chinese and English operation interface;

(24) Weight: ≤ 120 kg;

(25) Interfaces: 5 USB interfaces, 1 Ethernet interface, 1 serial port, and 1 high-definition display interface;

(26) Recovery concentration: 1.0E+11-13 Particles/ml

(27) Filtering shear force: less than 2000S-1, intact preservation of extracellular vesicles;

(28) Consumable packaging: irradiation sterilization packaging;

(29) Aseptic collection: End of pipe aseptic collection procedure improves yield;

(30) Concentration compression ratio: 10-200 times;

(31) Purification function: synchronous real-time purification, real-time removal of impurities and free dyes;

(32) Filter unit residue: Filter without any residual extracellular vesicles;

(33) Cloud based APP mini program: remote display of instrument operating environment, with alarm prompt function;

(34) Pump method: Disposable syringe, low cost of use;

(35) Concentration multiple setting: Initial concentration volume setting, real-time controllable;

(36) Concentration function: Independent two-stage concentration, convenient for process development;

(37) Filtering method: The dynamic rotatable filtering unit maintains stable internal pressure, improves the service life of the filtering unit, and enhances filtering efficiency;

(38) Cleaning function: alkaline solution, PBS circulation cleaning;

(39) Ultrasonic vibration: equipped with ultrasonic vibration mode to optimize the efficiency of extracellular vesicle filtration and recovery;

(40) Processing method: Independent synchronous operation

(41) Clean air efficiency: ≥ 99.99% @ 0.3 μ m; Pressure drop ≤ 130Pa;

quality assurance

Our company promises to provide a 5-year warranty for the entire machine (including vulnerable and consumable parts) and lifetime maintenance. The free warranty period starts from the date of acceptance, and a stamped written warranty commitment letter from the original factory must be submitted when signing the contract.