Field scanning confocal imaging system BrukerOpterra II

Field scanning confocal imaging system BrukerOpterra IIDue to its high resolution and excellent optical slicing ability, it has become an important tool for life science research. However, traditional point scanning methods suffer from slow imaging speed and high phototoxicity. The field scanning confocal microscope launched by Bruker adopts multi-point/slit scanning technology, combined with high-sensitivity detectors, to achieve high-speed and low phototoxicity imaging effects while maintaining high resolution, and is suitable for different magnification objectives, overcoming the limitations of traditional confocal microscopes.

Field scanning confocal imaging system BrukerOpterra IICompared to conventional fluorescence imaging equipment, it has higher resolution and good optical slicing ability, and has become a conventional instrument in life science research. However, commonly used point scanning laser confocal products have slow imaging speed and high phototoxicity, making them unsuitable for imaging live cells. Although its alternative product, the rotary confocal microscope, has improved imaging speed and phototoxicity, it suffers from severe fluorescence crosstalk and is not suitable for fluorescence imaging of thick samples; And the pinhole size on the turntable is fixed, which can only have confocal effect for a specific objective lens. To address this issue, Bruker has introduced a field scanning confocal microscope, which utilizes multi-point/slit scanning and high-sensitivity EMCCD/sCMOS detection to achieve high-speed and low light toxicity imaging without sacrificing image resolution, and can achieve confocal imaging with multiple magnification objectives.