F0193 fetal bovine serum

F0193 fetal bovine serum

Product Name:South American Uruguayan fetal bovine serum

Manufacturer:Sigma-Alldrich

Product Item Number:F0193

Product Specifications500ML

Product Features:

① Uruguay origin, with traceability

② Aseptic cardiac puncture for blood collection

③ Three sterile filters of 0.1 μ m

④ Strictly implement QC standards for multiple tests such as microbiology, endotoxins, hemoglobin, etc

⑤ Verified through cell culture

Advantages of fetal bovine serum:

1. Nutrients: Fetal bovine serum provides various essential nutrients for cells.

2. Growth factors: promote cell growth and proliferation. 3. Antibodies: help cells resist the invasion of certain pathogens. 4. Reduce toxicity: Some components of the culture medium may be toxic to cells, and other components in fetal bovine serum can help neutralize or reduce these toxicities. 5. Promote cell-cell interactions: Certain components in fetal bovine serum can promote cell-cell interactions.

3. How to thaw and replace fetal bovine serum? Generally, it is frozen at 20 ° C and melted between 2 ° C and 8 ° C. It is best to store large packages in small packages according to the required quantity each time to avoid repeated freezing and thawing. Sedimentation often occurs after serum melting, which may be caused by the aggregation of serum proteins. These precipitates generally do not affect serum quality and may hinder microscopic observation of cells. They can be briefly removed by centrifugation. The internationally popular method for melting serum is the three-step method. Taking 500 mL of serum as an example, it is left at room temperature for 1.5 hours, in a 20 ° C water bath for 25 minutes, and in a 37 ° C water bath for 35 minutes. Adhering to the principle of "using and preparing on the spot", fresh culture medium with added serum and antibiotics is recommended to be used up within one week. When it is necessary to replace the brand of fetal bovine serum due to supply, cost-effectiveness, poor cultivation effect, or when long-term use of one brand leads to domestication of cells and poor cultivation effect, it is particularly important to smoothly pass the transition period. It is advisable to first try replacing serum from the same source, followed by replacing serum from different blood sources. It is recommended to try the proportion replacement method, which involves 2-3 stages of replacement, gradually reducing the proportion of the original brand. Just replacing the serum may slow down cell proliferation. It is recommended to observe and judge after 3 or more passages per stage/replacement.

4. Australian fetal bovine serum Due to its geographical advantages such as grassland climate, strict breeding environment, and disease control, Australia is a well-known producer of fetal bovine serum. Taking serum from New Zealand as an example, Australian fetal bovine serum usually has good quality and a wide range of applications, which can meet various cell culture needs such as conventional primary cells, stem cells, difficult to culture passage cells, immune cells, tumor cells, etc.

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Therefore, choosing the right and reliable fetal bovine serum and manufacturer is extremely important↓↓↓↓

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F0193 fetal bovine serumA more cost-effective optionWinsera fetal bovine serum:

WinSera ® Characterized FBS Fetal Bovine Serum FBS500-WS-002 500ml

WinSera ® Characterized FBS Fetal Bovine Serum FBS50-WS-001 50ml

WinSera ® Premium fetal bovine serum (boxed) Fetal Bovine Serum FBS50-WS-001 50ml*10

WinSera ® Defined FBS Fetal Bovine Serum FBS500-WP-002 500ml

WinSera ® Defined FBS Fetal Bovine Serum FBS50-WP-001 50ml

WinSera ® Premium fetal bovine serum (boxed) Fetal Bovine Serum FBS50-WP-001 50ml*10

WinSera ® Stem cell specific fetal bovine serum Stem cells Qualified FBS500-WE-002(ES) 500ml

WinSera ® Stem cell specific fetal bovine serum Stem cells Qualified FBS50-WE-001(ES) 50ml

WinSera ® Stem cell specific fetal bovine serum Stem cells Qualified FBS50-WE-001(ES) 50ml*10

WinSera ® No extracellular vesicle serum Exosome-depleted FBS FBS050-EXO 50ml

WinSera ® AB serum Human Serum AB HU050-S-011 100ml

WinSera ® Heat inactivated fetal bovine serum Heat Inactivated FBS FBS500-WH-002 500ml

WinSera ® Carbon adsorption of fetal bovine serum Dextran Stripped FBS FBS500-WCS-002 500ml

WinSera ® Dialysis of fetal bovine serum Fetal Bovine Serum, Dialyzed FBS500-WDF-002 500ml

WinSera ® Ultra low IGg fetal bovine serum Low IgG FBS FBS500-WLG-002 500ml

WinSera ® Newborn bovine serum NewbornCalfSerum NCS500-W-002 500ml

Analysis of common problems with fetal bovine serum:

What is serum precipitationSerum is not an artificial product, and precipitation is a natural phenomenon. The precipitation is mainly fibrinogen, followed by salt precipitation. Such as calcium phosphate, as well as some steroids, fatty acids, and other protein precipitates

What factors increase serum precipitation? Serum heat inactivationFactors such as culturing at 37 ℃, repeated freeze-thaw cycles, failure to shake well during dissolution, exposure to gamma rays, long-term storage at 2-8 ° C, addition of serum to RPMI 1640, high pH, evaporation of culture medium in culture plates or dishes can all lead to an increase in precipitation.

Precipitation does not affect its growth promoting effect on cells. Technical personnel have conducted in-depth research on this issue and have proven that serum precipitation has no effect on cell growth, but there is one exception: precipitation may have some impact on macrophage culture.

Precipitation occurs in all serum samplesG * brand fetal bovine serum does not undergo pre aging. If stored at 2-8 ℃, various proteins or esters in the serum may coagulate and precipitate, resulting in sedimentation or turbidity This will not affect the growth promoting effect of serum on cells We suggest storing the serum at -20 ℃ and avoiding repeated freeze-thaw cycles. The S * brand fetal bovine serum instruction manual states: "During the melting process, turbidity and flocculent precipitation occur, which is normal for most sera and does not affect their quality of use. However, it affects the appearance of the serum and the consistency between bottles.

The presence of sediment in the serum is not a sign of contamination. A better method for detecting serum contamination is to inoculate the serum onto a agar plate and culture it to see if bacterial colonies have formed. inUnder a 1000x microscope, experienced individuals can identify whether serum is contaminated by observing the state of cell fluid and the appearance of common bacteria, or by detecting the presence or absence of Gram stained bacteria.

How to remove sediment from serumTo remove these flocculent precipitates, the serum can be divided into sterile centrifuge tubes and centrifuged at 400g. The supernatant can be directly added to the culture medium for use. Do not remove these flocculent sediments by filtration, as it may clog the filter membrane.

How to reduce precipitation in the process of serum dissolution? After removing the serum from the freezer, place it inDissolve it in a refrigerator at 2-8 ℃ for about 12-24 hours, and then fully dissolve it at room temperature. However, it must be noted that during the dissolution process, it must be shaken gently and evenly in a regular manner. Do not directly place the serum just taken out of the -20 ℃ refrigerator in a water bath, whether it is room temperature water or 37 ℃ water, because the serum quickly melts in the water bath, and the large temperature difference (-20 ℃ to 37 ℃, with a temperature difference of 57 ℃) can easily cause serum precipitation. Directly placing it in a 65 ℃ water bath is an extremely cruel practice, which is a violation of serum and a rough trampling of scientific rules!

Precautions for fetal bovine serum cryopreservation: Serum that needs to be stored for a long time should be stored in-In a low-temperature refrigerator between 20 ℃ and 70 ℃. Do not store in a refrigerator at 4 ℃ for more than one month. Due to the volume increase of about 10% when serum freezes, it is necessary to reserve a certain volume of space before freezing it into a low-temperature refrigerator, otherwise contamination or glass bottle freezing may occur. The freezing of large packaged bottled serum requires a gradual freezing method: the serum in a low-temperature freezer at -20 ° C to -70 ° C is dissolved in a 4 ° C freezer. Then move to room temperature and wait for all to dissolve before packaging. During the dissolution process, it is necessary to constantly shake it quietly and evenly (be careful not to form bubbles) to ensure uniform temperature and composition, and reduce the occurrence of sedimentation. Do not directly melt the serum from -20 ℃ to 37 ℃. Under large temperature differences, the serum is prone to protein aggregation and sedimentation, resulting in flocculent substances.