Ex vivo muscle testing system

1500A：Ex vivo muscle testing system

Adapting microscope

The standard system configuration of 1500/1510/1530A includes everything needed to start testing immediately: length controller, force sensor, stimulator, force sensor connected to muscles through a slot at the end of the bath, including XYZ micrometer platform with built-in bracket. This provides an ideal configuration for artificial muscle structure or oxygen consumption research, which requires sealed chambers with lids.

The system can be used in conjunction with most inverted microscopes, allowing for integrated measurement of sarcomere length and bioluminescence. FluoroTrack and HVSL/VSL video sarcomere length modules are paired for real-time synchronous measurement of force, calcium, and sarcomere length.

Product Features

Capable of characterizing small and complete muscle tissue, performing all standard measurements including convulsions, tetanus, fatigue, length tension, force frequency, force velocity, stiffness, and work cycle. Signal acquisition and data management are processed by software, including standardized protocols, which can transform complex experiments into simple and direct measurements.

●AllowControl and measure force and length●Temperature controlled bath board size 400-1900 µ L

●Compatible with standard and inverted microscopes●Resolution as low as 0.01 μ N

●Power range from0.5-1000 mN ●A complete testing system with integrated bath device, suitable for complete small muscles

System Composition

Application Examples

According to the sample:

■Skeletal muscles:

Living body - extensor digitorum longus (EDL), soleus muscle, plantar muscle, diaphragm, vermicularis muscle

In situ - tibialis anterior (TA), gastrocnemius, quadriceps femoris, hamstring, tongue, rotator cuff

Off body dorsiflexion (TA, EDL), plantar flexor muscles (stomach, flounder, sole), mandible, index finger (flexor digitorum brevis FDB)

■Artificial muscles and connective tissues: cartilage, epithelial tissue, tendons.

According to the type of experiment:

▲ Twitch: A single pulse designed to induce single or small muscle fiber contractions.

▲Ankylosing contraction: rapid and continuous electrical pulses that causeMuscle contraction.

▲Fatigue: Frequently repeating the largest number of rigid contractions to cause muscle fatigue.

▲Force frequency: The rate at which the stimulation frequency is changed to evaluate the optimal frequency that causes the maximum tetanic force.

▲Irregularity: Actively stretching muscles during isometric rigid contractions to induce injury and evaluate resistance to injury or recovery from it.

▲Gait analysis/modeling exercise (isotonic, concentric): Control muscle strength output (isotonic) to evaluate muscle shortening speed.

▲Stiffness: Passive sine extension and muscle shortening are used to evaluate the intrinsic stiffness of tissues.

▲Stress strain: Incremental elongation of the tissue is used to calculate the Young's modulus of the material.

Video Introduction

References

1. Powers et al.“Subcellular Remodeling in Filamin C Deficient Mouse Hearts Impairs Myocyte Tension Development during Progression of Dilated Cardiomyopathy”International Journal of Molecular Sciences (2022)

2. Schick et al.“Reduced preload increases Mechanical Control (strain-rate dependence) of Relaxation by modifying myosin kinetics”Archives of Biochemistry and Biophysics (2021)

3. Laurila et al.“Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy”Science Advances (2022)

4. Blitz et al.“Infiltration of intramuscular adipose tissue impairs skeletal muscle contraction”The Journal of Physiology (2020)

5. Hall et al.“Cellular rescue in a zebrafish model of congenital muscular dystrophy type 1A”NPJ Regenerative Medicine (2019)

Aurora Scientific

For over thirty years, Aurora Scientific has been an ideal choice for scientific researchers around the world. Dual mode lever systems and force sensors are widely used in life science research disciplines, including sports and metabolism, cardiology, injury recovery, gerontology, disease progression, pharmacology, and more. Rapid response sensor products are considered as benchmark instruments for studying odor dynamics and olfactory studies of pheromone/plume dispersion; Mechanical stimulator technology is used to study the response of neurons to touch, pain, and stretching. The various types of testing systems produced by the company are used by materials science researchers to study the properties of artificial muscles, sports, and nanomaterials.