Domestic fully automatic amino acid analyzer

1、 Product development background:

(1) L-3000 typeDomestic fully automatic amino acid analyzerIt is designed based on the post column derivatization principle of indene ketone, and is divided into hydrolyzed protein analysis system (sodium salt) and physiological fluid analysis system (lithium salt), which comply with national standards such as GB5009.124-2016 "Determination of Amino Acids in Food", "Determination of Amino Acids in Feed", and "Calibration Regulations for Amino Acid Analyzer".

(2) To meet the needs of amino acid analysis, our company adopts SHU liquid pump, automatic sampler, detector, post column derivatization system and other components specially designed for amino acid analysis, which effectively improve the separation, detection limit and repeatability of amino acid analysis.

IIDomestic fully automatic amino acid analyzerTypical clients include Qinghai Normal University, Guangxi University of Traditional Chinese Medicine, a agricultural and sideline product processing project (feed) at a university in Qingdao, a third-party testing company (water-soluble fertilizers and feed) in Jining, Shandong, Guangdong Xinde Biotechnology (health products), Xiamen University, Tianjin Ruipu Biotechnology Co., Ltd., Huazhong Agricultural University, a national agricultural science and technology park project in Hunan, and Hunan Xianweikang Biotechnology, among others.

3、 Characteristics of domestic amino acid automatic analyzer:

1) The post column derivatization method using indene ketone meets the standards and national standards.

2) The hardware consists of two independent parts: the host and the post column derivative system. The host can be used as a standalone quaternary gradient liquid chromatography system, with high equipment utilization.

3) The automatic sampler is equipped with an ammonia elimination system, effectively avoiding the impact of ammonia on the baseline.

4) Specially designed high-temperature ultraviolet detector.

5) The post column derivative system, which has been repeatedly optimized, effectively reduces the dead volume and pressure fluctuations of the system.

6) Low maintenance costs: The column has a long lifespan, and the prices of reagents and consumables are comparable to other imported brands of amino acid analyzers.

4、 Main parameters of domestic amino acid automatic analyzer:

1 pump

1) Four element low-pressure gradient pump with pulsation below 1% or 0.1Mpa

2) Flow rate range: 0.001ml/min-10.000ml/min

3) Flow accuracy: ± 0.2%

4）梯度精度： ≤0.1%(JJG)≤0.06%RSD(ASTM)

5) High withstand voltage of 9000Psi

6) The plunger rod has automatic cleaning function, anti salt precipitation, stable performance, and long service life.

7) Built in four channel online vacuum degassing machine

8) Material: SUS alloy lining or titanium alloy lining.

2 Automatic Sample Injectors

1) Injection method: It has both ring injection and variable injection modes.

2) Injection volume: 1ul -500ul

3) Reproducibility: The variation is less than 1% when injecting 10ul variable volume samples.

4) Memory effect:<0.01%.

5) Sample tray: Two trays, each containing 48 sample grades and 1.5ml sample bottles.

3 separation units

1) Chromatographic column: Sodium ion exchange column, 4.0 * 150mm (imported)

2) Ionic column protection column component

4 Derivative Units

1) Derivative method: Indene ketone post column derivatization method

2) Temperature setting: Room temperature~150 ℃ (increments: 0.1 ℃).

3) Gradient heating program improves separation and running time.

5 detectors

1) Spectral system: superior to filter based spectral analysis, with aberration eliminating concave diffraction grating for spectral analysis.

2) Detection wavelength: 190-800nm

3) Equipped with a reference channel, the detection wavelength is constantly calibrated.

6 Chromatography Workstation

Capable of controlling instruments, collecting and processing data. It can achieve automatic cleaning of the system, comply with GLP/GMP regulations, conduct system verification tests, customize customer reports, and user safety verification.