DSI BUXCO animal oral and nasal exposure inhalation toxicity system

Experimental animal exposure poisoning system

Oral and nasal precise exposure tower - Inhalation Tower

Buxco ® Inhalation Tower， The precise exposure tower for mouth and nose is suitable for inhalation exposure and toxicity research, supporting various media such as dry aerosols, dust, smoke, steam, etc. The tower has a total of six layers, with seven exposed ports on each layer. Different layers of the tower can be stacked in any combination as needed, and exposure experiments can be conducted on up to forty-two animals simultaneously.

The internal structure of the exposed tower is shown in the figure above. The top view cross-section consists of two concentric circles, which are two concentric cylinders nested together. The exposed material is input from the top of the exposed tower (blue arrow), enters the inner cylinder, and is sent to the animal's mouth and nose through the circular tube on the cylinder wall. The gas exhaled by the animal enters the outer cylinder and is pulled upward by negative pressure to flow to the outlet for discharge (red arrow).

Applicable species: mice, rats, guinea pigs, ferrets

Characteristics of precise exposure tower for mouth and nose:
Stainless steel material: The ultra smooth surface greatly reduces sample loss, has strong corrosion resistance, and is more suitable for harsh chemical environments such as Autoclave and other cleaning methods.
Stacked modular design of tower body: flexible configuration, easy operation, convenient disassembly and cleaning.
• Concentric circle design and Flow path special airflow channel design: avoids animals inhaling and exhaling gases, isolates the exposed medium from the exhaled gases of experimental animals, and ensures consistent concentration of the exposed gas environment
It can also reduce CO2 concentration.
Fully enclosed system: can control the pressure and flow rate inside the tower, and the negative pressure environment inside the tower avoids the leakage of exposed media. After verification, the concentration difference between each layer of the exposed tower is within 5%.

SmartStudy ™ Technology:


The accuracy and reliability of experimental results on inhalation exposure are crucial for preclinical drug testing, efficacy evaluation, and safety assessment. DSI expert team, after years of dedicated research, for Buxco ® The inhalation exposure poisoning platform has developed multiple cutting-edge technology designs, which can monitor the cumulative inhalation amount and exposure duration of aerosols in multiple dose groups of target animals in real-time, automatically, and accurately. When the target animal reaches the preset target dose or exposure duration, the system will automatically end the exposure and deliver fresh air without manual detection and intervention. Animals that reach the target dose will continue to stay in the chamber to breathe fresh air, without affecting the continued exposure experiment of other animals.
SmartStudy ™ Technical advantages:
• Accurate and reliable - Once the target dose is reached, the system will automatically stop exposure
• Convenient and worry free - fully automated detection and monitoring, professional calculation, induction, summarization, and report generation
• Safety and environmental protection - efficient use of animal resources, improving animal welfare while avoiding environmental pollution, ensuring the safety of laboratory personnel
• Flexible and efficient - Stacked modular design, capable of conducting multiple dose group trials simultaneously

Allay ™ Exposed cavity
Using Allay ™ The Allay card designed with technology can fix animals to maintain their natural breathing posture and state throughout the experiment,
Eliminating many adverse effects caused by compression of animal chest, abdomen, and respiratory tract due to various traditional exposed cavities.
Allay cards come in various sizes and are suitable for multiple animal species. They can shorten the average animal loading time to less than 30 seconds, saving nearly half of the time compared to traditional methods.


• Reduce exposure medium consumption - Under standard test conditions, the data validity of DSI exposure tower tests can reach 70-85%
• Reduce animal resource consumption - significantly reduce unnecessary exposure and animal consumption; Achieve precise target dose exposure without the need for traditional high-dose group trial protocols
A single exposure tower can run multiple dose group tests simultaneously, with automatic self checking of the entire system, saving time, effort, and worry

Exposed tower main system controller

Push/Pull can control the pressure environment inside the tower, creating positive or negative pressure
Status, avoid exposure to medium leakage
• Supports regulation and calibration of up to 4 nebulized drug delivery heads
There are 4 signal amplifier interfaces, paired with FinePointe software and
The respiratory plethysmography chamber can monitor tidal volume, minute ventilation, and respiratory rate
Various physiological indicators of respiratory function such as inhalation frequency
Paired with FinePointe software and corresponding accessories, it can monitor the airflow inside the tower
Air pressure, temperature and humidity, 02/CO2 concentration ratio, exposure medium particle concentration
Degree, etc., to expand more possibilities for exposure experiments

Auxiliary Controller for Exposed Tower System

Used for functional expansion of the main controller, it can be used independently with an exposure tower or a single animal mouth and nose exposure device.
• Supports up to 8 signal amplifier interfaces
• Supports regulation and calibration of up to 4 nebulized drug delivery heads
• Can monitor dual channels 02/C02 inside the tower
• Perform gas analysis and calibration
• Support media mixing control

Single Site Exposure Station - Single Animal Mouth and Nose Exposure

The economical and applicable version of the precise exposure tower system for mouth and nose is an ideal choice for each experiment that only requires a single experimental animal to be exposed for related research. Supports various media such as dry wet aerosols, dust, smoke, steam, etc., and is compatible with Aerogen Aeroneb atomization head for drug delivery. It can also be used in exposure experiments with a respiratory volume recording chamber to achieve synchronous monitoring of respiratory function.

Characteristics of single animal mouth and nose exposure system:
• Apply Allay ™ Exposure of nasal cavity and exposure tower in technical design
Same cavity, both can be used interchangeably
Each device only supports exposure of a single experimental animal at a time
Suitable for various experimental animal species such as mice, rats, guinea pigs, and ferrets

Whole body exposure of group animals - Mass Dosing

The exposed chamber top cover is a movable non sealed design with four atomization or gas input interfaces. If equipped with a smoke generator, it can input first-hand smoke and second-hand smoke separately. The cavity is equipped with a porous bottom plate, which helps with urine discharge and improves animal welfare. Multiple isolation plates can be placed as needed to maintain an appropriate distance between experimental animals. The chamber has four air supply interfaces, which can be connected to devices such as deflectors. If you need to obtain accurate nasal exposure data, please choose the Inhalation Tower system.

Standard version: up to 15 mice or 6 rats, or 2 guinea pigs, 24.77 × 27.36 cm
Extended version: up to 25 mice or 10 rats, or 4 guinea pigs, 34.29 × 36.88 cm
The number of animals that can be accommodated in the cavity depends on the weight and age of the experimental animals

Suitable for experimental research on OVA nebulization sensitization in asthma modeling, as well as the establishment of COPD models through smoking in combination with smoke generators
Simultaneously conducting group full body exposure experiments with multiple experimental animals, adapting and supporting up to four Aerogen Aeroneb nebulization heads for simultaneous nebulization

Exposure Source Generators

EVT Universal Smoking Machine - E-cigarette, Vape, and Tobacco

The EVT universal smoking machine is suitable for preclinical nebulization experiments of various cigarette sources such as traditional cigarettes and smoke vaporizers. The software is designed for intelligent self checking, which optimizes the operation process to a greater extent and assists users in conducting various research more efficiently. It can customize multiple parameters such as suction frequency, suction duration, suction capacity, and real-time monitoring of accumulated smoke volume, suction frequency, smoke concentration, and experimental animal inhalation volume. EVT can be paired with inhalation exposure exposure systems, whole-body volume recording systems, or respiratory function testing platforms to expand more functions and applications, such as in vitro cell exposure, smoke content, chemical analysis, as well as precise exposure of experimental animals' mouths and noses and lung function testing, providing you with more comprehensive and reliable experimental data and diversified one-stop solutions.

Each EVT can be equipped with 3 workstations and perform atomization experiments on 3 different types of smoke sources simultaneously, significantly reducing experimental variability.
Supports high-throughput experiments, flexible and diverse operations, and can merge the smoke generated by three workstations to generate high concentration smoke.
Paired with a smoke collection tube, it can collect second-hand smoke.
Smoke Bellows ® technology
You will no longer need to spend a lot of time and energy on equipment cleaning and maintenance:
Each Bellows can support up to 10000 puffs and ensure that each puff meets industry ISO standards.
The software can automatically detect whether Bellows is installed properly, the frequency of Bellows usage, and remind users to replace it in a timely manner.
Ensure that all smoke is isolated from the equipment itself, avoiding unnecessary pollution while also protecting the equipment and extending its lifespan.

Automatic Cigarette Smoking Machine - Multiple Cigarette Smoke Generator

The integrated fully automatic cigarette smoking equipment that works independently can operate continuously for 8 to 24 hours without the need for manual supervision. After loading the cigarette, simply press the "Start" button to automatically load the cigarette into the nozzle, ignite it, and automatically spit out the cigarette butt. At present, there are standard and extended versions available. The standard version can hold 80 cigarettes, while the extended version can hold 300 cigarettes.

Features of fully automatic cigarette smoking machine:
Adopting the industry standard "artificial lung" to simulate human smoking patterns, with independent "first-hand smoke" and "second-hand smoke" channels, it can be transported to whole body violence in group animals
An exposed cavity or other separate exposed channel.
Equipped with built-in lighting, the situation inside the cigarette combustion chamber can be observed through the glass window.
• Can set inhalation volume, inhalation duration, number of puffs per minute, number of minutes per cigarette, ignition interval between cigarettes, and total inhalation time
Parameters such as how many cigarettes to light.
The combustion chamber door adopts a pressure sealed design to ensure that smoke will not leak.
The compact integrated design is suitable for small laboratories and equipment such as standard fume hoods or biosafety cabinets.

Aerosol Generator - Aerogen Nebulizer Delivery Head



The Aerogen atomizing drug delivery head can generate the exposed medium into aerosol form, with two specifications available: 2.5-4 μ m and 4-6 μ m
• Strong versatility: it can deliver all drugs approved to be used for standard spray to treat infants to adult patients; The drug room capacity is up to 10ml, which can reach 17% of lung deposition, while the traditional small volume spray is only 3%.
• Easy to operate: The atomization head control system is integrated into the exposure tower main controller, auxiliary controller, and group animal whole-body exposure controller, which can adjust its own atomization efficiency and set the atomization amount and time through software and hardware.
Economical: Reusable, supports high temperature and high pressure disinfection at 132-135 ℃.

Aerosol Generator - Collision Jet Nebulizer, PARI Nebulizer

The Collision Jet atomizer is used for efficient atomization of solutions or suspensions, with a wide range of atomization quantities:
Both vertical and horizontal atomization can be supported.
Equipped with safe and durable polymer containers, suitable for handling small capacitiesrareOr high-value liquids.
• Stable performance, standardized operation, and support for long-term continuous operation.
Adapt to harsh chemical environments (such as high-pressure steam cleaning and other cleaning methods). PARI atomizer, suitable for long-term nebulization inhalation experiments:
Fewer components, vertically installed to prevent incorrect operation.
Equipped with an intermittent control button, the medication can be continuously or intermittently output.
• The spray is equipped with a valve device, so the treatment time is short and the sample waste is less.
Two different cores to meet various applications
Equipped with a PIF control system (peak inspiratory flow rate control system), the inhalation airflow is limited to no more than 25L/min. In a controllable mode, it helps practice deep and slow inhalation and facilitates effective drug deposition.

Other optional items for the DSI Buxco platform include:
Pre treatment of target exposure environment concentration inside the tower, valve adjustment, and drying equipment
Environmental monitoring - temperature and humidity monitoring, O2/CO2 concentration ratio monitoring
• Particle weighing and analysis - concentration monitoring, particle size monitoring, weight detection
If researchers have a need for environmental treatment, monitoring, and particle weighing and analysis as mentioned above, please consult the company's pre-sales support for details.