DNA RNA co extraction kit

DNA RNA co extraction kit

DNA RNA co extraction kitThis kit is suitable for rapidly extracting high-purity viruses, bacteria, fungi, etc. from various samples such as blood, tissues, and fecesDNA/ RNAThe reagent kit adopts a sample processing scheme, combined with nucleic acid protectants, which can significantly improve the recovery rate of trace nucleic acids and strictly control the extraction process. Through the specific adsorption of silica gel membrane, viruses and bacteria can be quickly and efficiently purified Fungi DNA/RNA。 The sample has wide compatibility, high yield and purity of nucleic acid obtained, and can be directly used for reverse transcription PCR、 LumineLight quantificationPCR, etcDownstream related experiments.

The sample needs to be preprocessed in advance, which is approximately required2 hours, the preprocessing steps are as follows:

1) Grind with a grinder for about 10 minutes

2) Metal bath at 37 ℃ for 1 hour and 30 minutes

3) Add proteinase K and metal bath at 56 ℃ for 10 minutes

The following processes are all carried out in a biosafety cabinet1. Add 300 μ l sequentially to the RNase free tube Buffer lysis buffer, 25 μ l nucleic acid protectant, 300 μ l Bufferpre-processingLiquid,Vortex mixing15-30sec， Collect the liquid on the cover and wall of the tube by centrifugation briefly, and let it stand at room temperature for 5 minutes.

2.join200 μ l anhydrous ethanol, vortex and mix for 15-30 seconds, briefly centrifuge for collectionLiquid on the manifold cover and pipe wall.

If there is impurity precipitation at this time, it is a normal phenomenon, and subsequent experiments can be carried out directlyTest.

3. Transfer all the above mixture to DNA/RNA Columns (DNA/RNA Columns have been placed in the collection tube),Centrifuge at 12000 rpm (13800 × g) for 1 minute and discard the filtrate.

4. Towards DNA/RNA Add 700 μ l to Columns Buffercleaning solution1 (Please)First check if it has been addedabsolute ethanol)，Centrifuge at 12000 rpm (13800 × g) for 30 minutes sec， Discard the filtrate.

5. Towards DNA/RNA Add 700 μ l to Columns Buffercleaning solution2 (Please check if anhydrous ethanol has been added first),Centrifuge at 12000rpm (13800 × g) for 30 seconds,Discard the filtrate.

6.12000rpm (13800 × g) empty column centrifugation 2min。

7. Be careful when handlingDNA/RNA ColumnsTransfer to a new oneRNase-free Collection Tubes 1.5ml(Reagent kit provided)Middle, suspend and add to the center of the membrane30-50 μ lRNase-free ddH2O，Let it stand at room temperature1min，12,000rpmCentrifuge at 13800 × g for 1 minute.

8.discardDNA/RNA Columns， The extracted DNA/RNA can be directly used for subsequent testing. For short-term storage, please store it at -30~-15 ℃, please store at -85~-65 ℃ for long-term storage.