DMEM/F12 (sugar free) basic culture medium

DMEM/F12 (sugar free) basic culture mediumIntroduction:

Basic components:

Cell culture medium mainly consists of three parts: basic culture medium, additives, and growth factors.

Basic culture medium is the most important component of cell culture medium. It is a basic liquid culture medium typically composed of water, amino acids, sugars, vitamins, and minerals to provide sufficient nutrients and growth conditions to maintain cell survival and proliferation. Common basic culture media include DMEM, MEM, RPMI-1640, etc.

Additives mainly include fetal bovine serum (FBS), human serum, sheep serum, chicken embryo serum, horse serum, soy protein hydrolysate, etc. These additives provide many substances and growth factors that cells need to help with cell growth and proliferation. FBS is one of the additives commonly used for culturing tumor cells and other difficult to culture cells. The addition of FBS can enhance cell adhesion, proliferation rate, and inhibit cell apoptosis.

Growth factors are one of the important components in cell culture media, which can promote cell proliferation, differentiation, and functional expression. Growth factors include EGF, FGF, NGF, PDGF, etc. These growth factors regulate cell proliferation and differentiation by activating corresponding receptors. Growth factors have different effects in different cell types, so different cell types require different growth factors.

Classification of cell culture media:

According to their composition and function, cell culture media can be divided into two types: serum-free media and serum containing media.

Serum free culture medium is a cell culture medium that does not contain FBS or other animal serum. Due to certain drawbacks of animal serum, such as the presence of unknown growth factors and batch instability, serum-free culture media have gradually been widely used in cell culture. Serum free culture medium is usually composed of specific combinations and concentrations of growth factors, basic culture medium, additives, and some colloids or polymers. The use of serum-free culture medium can improve the stability, reproducibility, and accuracy of cell growth and proliferation, reducing potential issues such as biological contamination and immune reactions.

Serum containing medium is a cell culture medium that contains animal serum (usually FBS) and is one of the traditional cell culture media. FBS plays many important roles in cell culture, such as providing essential growth factors, promoting cell proliferation and adhesion, and inhibiting cell apoptosis. However, due to the uncertainty of FBS sources and batch variations in composition, serum containing culture media may have issues of instability and non repeatability in certain situations. In addition, when using serum containing culture medium, it is necessary to screen and process the serum to remove potential pathogens and pathogenic bacteria.

The products currently being sold by the company:

Notes:

1. When using the product, attention should be paid to aseptic operation to avoid contamination.

2. This product contains serum and bispecific antibodies. If there is no special need, there is no need to add additional serum and bispecific antibodies, and they can be used directly.

3. To maintain the best performance of this product, it is not advisable to place it in room temperature or higher temperature environments for a long time.

4. All products should be used within the shelf life. If they exceed the shelf life, they must be discarded.

5. This product is only for further scientific research use and cannot be applied to clinical or other aspects.