DBCO-NH-PEG4-Biotin ， Small molecule PEG

Common names:DBCO-NH-PEG1-CH2CH2COONHS Ester， Small molecule PEG

Description:

DBCO-NH-PEG1-CH2CH2COONHS Ester， Small molecule PEGIt is a polyethylene glycol (PEG) derivative with a double alkynyl epoxy (DBCO) group, amino (NH) linked to four ethylene glycol units (PEG4), and labeled with Biotin. This compound is commonly used in applications such as biomolecular labeling, immunoassay, protein conjugation, and bioimaging. It combines Biotin's high affinity with the click chemistry reaction ability of DBCO groups, enabling efficient click chemistry reactions with molecules containing azide groups.

molecular structure

1. DBCO (diynyl epoxy) group

• The DBCO group is an important functional group in click chemistry that can react with azide groups to form stable 1,2,3-triazole rings.

• Its high reactivity makes it a key component in biomolecule coupling and labeling, suitable for live cell labeling and various protein coupling reactions.

2. PEG4 chain

• PEG4 is a polymer chain composed of four ethylene glycol units, providing water solubility, low immunogenicity, and good biocompatibility.

• The short chain length of PEG4 ensures molecular flexibility and high solubility, while reducing interference with the functionality of biomolecules.

3. Biotin

• Biotin is a small molecule vitamin that has an affinity for streptavidin or avidin.

• Biotin is commonly used for biomolecule labeling, immunoassay, and biomolecule capture experiments.

Main properties

1. High affinity and selectivity

• The Biotin group has a very high affinity for binding to streptavidin or avidin, making it suitable for molecular capture and targeted analysis.

2. Efficient click chemistry reaction

• DBCO groups can react with azide groups to rapidly and specifically link molecules together through click chemistry reactions, forming stable 1,2,3-triazole ring structures.

3. Good water solubility and biocompatibility

• Due to the introduction of PEG chains,DBCO-NH-PEG4-BiotinIt has good water solubility and can exist stably under biological conditions. The PEG chain reduces immunogenicity and enhances biocompatibility.

application

1. Biomolecular markers

• Biotin groups can bind to streptavidin or avidin for labeling and detecting specific biomolecules.

• Commonly used methods in immunological experiments include immunoprecipitation, Western blot, ELISA, etc.

2. Molecular coupling and drug delivery

• DBCO groups can react with azide groups for molecular coupling, and are widely used in the construction of drug delivery systems, combining drug carriers or other functional molecules with biomolecules.

• The Biotin portion can be used to enhance the targeting of conjugates.

3. Biological Imaging and Probes

• By combining fluorescent dyes or other imaging probes, targeted labeling and cell imaging can be achieved through the Biotin avidin system.

4. Protein coupling and surface modification

• This compound can be used for coupling proteins, peptides, or other molecules with surface modifications, enabling the construction of protein chips and functionalization of biosensors.

Reaction conditions and purification

1. coupling reaction

• When DBCO groups react with azide groups, they are usually carried out under mild conditions, often using DMSO or aqueous buffer as solvents.

• The binding of Biotin groups to streptavidin or avidin occurs under neutral or weakly alkaline conditions, typically in PBS or other biologically adapted buffer solutions.

2. Purification method

• Dialysis, gel filtration, reverse phase high performance liquid chromatography (RP-HPLC) and other methods can be used for purification to remove unreacted reagents and by-products.

Storage and operation precautions

1. store

• This compound should be stored in a dry, cool place to avoid the effects of high temperature and moisture.

• For long-term storage, it is recommended to store it at -20 ° C to maintain stability.

2. operation

• Proper protective equipment should be worn during operation to avoid direct contact with chemical reagents.

• Avoid excessive exposure to light and humid environments during the reaction process to ensure the stability of the compound.

main advantages

1. Efficient coupling capability

• Efficient and selective coupling reactions can be achieved through the click chemistry reaction between DBCO groups and azide groups, which is suitable for biomolecule modification and labeling.

2. Biotin's high affinity

• Biotin groups can bind with streptavidin or avidin to form highly stable complexes, suitable for biological labeling and molecular capture.

3. Good water solubility and biocompatibility

• The introduction of PEG chains gives the compound good water solubility and biocompatibility, reducing immune reactions and making it suitable for biomedical applications.

DBCO-NH-PEG1-CH2CH2COONHS Ester， Small molecule PEG It is a biomarker reagent with efficient coupling ability, widely used in fields such as biomolecular labeling, immunoassay, drug delivery systems, surface functionalization, etc. It combines the excellent water solubility and biocompatibility of PEG chains, as well as the high affinity of Biotin with avidin systems, making it an important tool in the research and application of biomolecules.

Purity: 90%+

Packaging: Bottled!

Origin: Xi'an

Purpose: Scientific research!

Specification: mg/g

Kind reminder: For scientific research only, not for human experiments!

About us: Xi'an Huirui Biotechnology's main business directions include synthetic phospholipids, polymer polyethylene glycol derivatives, block copolymers, paramagnetic/superparamagnetic nanoparticles, nanogold and nanogold rods, near-infrared fluorescent dyes, reactive fluorescent dyes, fluorescently labeled dextran BSA and streptavidin, protein crosslinkers, small molecule PEG derivatives, click chemistry products, dendritic polymers, cyclodextrin derivatives, macrocyclic ligands, fluorescent quantum dots, hyaluronic acid derivatives, graphene or graphene oxide, carbon nanotubes, fullerenes, etc. We also provide a variety of customized product services!

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