- Phone
-
Address
B1, 9th Floor, Yangming Plaza, Xiaoying Road, Chaoyang District, Beijing
Beijing Bored Technology Development Co., Ltd
B1, 9th Floor, Yangming Plaza, Xiaoying Road, Chaoyang District, Beijing

10Minute rapid typing |Crystal ChemMouse monoclonal antibody typing kit
Cat#80619 (10T)
Introduction:
In the preparation of monoclonal antibodies, antibody engineering, and immunological research, mouse monoclonal antibodies/Accurate identification of subtypes and light chain types is a key step in hybridoma screening, clone validation, and antibody functional analysis. traditionELISAThe operation is cumbersome, time-consuming, and has low throughput,Crystal ChemlaunchRapidYield Mouse Monoclonal Antibody Isotyping KitUsing innovative nanoparticle lateral chromatography technology,10 Complete typing of mouse monoclonal antibodies in minutes, providing a minimalist, efficient, and reliable solution for scientific research and antibody development.
Product advantages:
1.Fast detection, efficient and time-saving
The entire process only requires10 Minutes, add samples-incubation-The interpretation is completed in three steps, without the need for washing or large instruments, greatly shortening the hybridoma screening cycle.
2.Subtype full coverage, precise and without omission
Simultaneous identification of heavy chain species in one experiment/subcategory+Light chain type, fully covering the mainstream types of monoclonal antibodies in mice.
3.Easy to operate
Sample by1:10or1:100After dilution, the test strip can be soaked and incubated, suitable for low abundance antibody samples with high sensitivity.
4.Strong sample compatibility
Support dual sample types of cell supernatant and mouse ascites, and adapt to the entire process of monoclonal antibody development.
5.Easy to operate:
Sample by1:10or1:100Dilute it, low concentration antibody (<10Mg/mL)It can also be detected stably.
6. Clear and reliable results
Equipped with a built-in quality control line, the red strip displays the type intuitively, and the results are accurate and reliable
Operation process:
3、 Experimental Procedure (Three Simple Steps)
1.Sample dilution
Take the cell supernatant and press1:100Dilution (concentration<10μg/mLPress and hold1:10Dilute and vortex thoroughly.
2.Test paper incubation
Insert the red and blue bars into the diluted sample, with arrows pointing downwards and markings pointing upwards, and let it stand at room temperature10–20minute
3.result interpretation
The color development of the quality control line is effective, and a red band appearing at the corresponding position indicates a positive type.
Product Information
brand |
Crystal Chem |
Product Name |
Mouse monoclonal antibody typing kit |
English name |
RapidYield Mouse Monoclonal Antibody Isotyping Kit |
Item Number |
80619 |
Testing Scope |
Heavy Chain:IgATheIgG1TheIgG2aTheIgG2bTheIgG3TheIgM Light chain: κ, λ |
Testing samples |
Cell culture supernatant and mouse ascites |
Detection Principle |
Nano particle lateral chromatography immunoassay with red bands for intuitive interpretation. |
Product Specifications |
Red detection strip10 Strip, blue detection strip10 Strip, diluent4mL, can be done10A sample |
Storage conditions |
2–8℃ refrigeration; The test strip can be stored at room temperature for a short period of time, and the diluent needs to be diluted 2–8℃保存 |
Product application:
1.Rapid screening of mouse hybridoma clones.
2.cell supernatant/Subtype identification of monoclonal antibodies in ascites.
3.Recombinant antibody expression validation and typing.
4.Confirmation of early development types of antibody drugs.
5.Immunological, infectious, inflammatory, and tumor model antibody response analysis.
For more product details, please inquireCrystal ChemBeijing Bored Biotechnology Co., Ltd. is the general agent for the China region.