- Phone
-
Address
B1, 9th Floor, Yangming Plaza, Xiaoying Road, Chaoyang District, Beijing
Beijing Bored Technology Development Co., Ltd
B1, 9th Floor, Yangming Plaza, Xiaoying Road, Chaoyang District, Beijing

10Quick identification in minutes |Crystal ChemHuman monoclonal antibody typing kit
Cat#80617 (10T)
Introduction:
In the preparation of monoclonal antibodies, antibody engineering, and immunological research, antibodies/Subclass and light chain typing identification is a crucial step in hybridoma screening, clone validation, functional research, and downstream applications. traditionELISAThe operation is cumbersome and time-consuming, making it difficult to meet the needs of high-throughput and rapid identification.Crystal ChemlaunchedRapidYield Human Monoclonal Antibody Isotyping Kit(Cat#80617)Relying on innovative nanoparticle lateral chromatography technology,10 Quickly complete human monoclonal antibody typing in minutes, providing researchers with a minimalist, efficient, and reliable solution.
Product advantages:
1.Quickly generate results
The entire process only requires10 Minute, sample incubation and interpretation, without the need for washing, incubation cycles, and complex instruments, greatly improving experimental efficiency.
2.comprehensive coverage
Synchronize the completion of heavy chain classes in one experiment/subcategory+Dual dimensional identification of light chain type, fully covering the mainstream types of human monoclonal antibodies.
3.Easy to operate
The sample only needs to be pressed1:10or1:100Dilute, soak and incubate the test strips, even beginners can quickly get started.
4.The results are intuitive and reliable
The red specific band indicates the corresponding type and has a built-in quality control line(CCC)Ensure the effectiveness of the experiment and make the results clear and easy to judge.
5.Adapt to scientific research scenarios
Specially optimized for cell culture supernatant, compatible with low abundance monoclonal antibody samples, meeting the needs of hybridoma screening, clone identification and other scenarios.
Operation process:
3、 Experimental Procedure (Three Simple Steps)
1.Sample dilution
Take the cell supernatant and press1:100Dilution (concentration<10μg/mLPress and hold1:10Dilute and vortex thoroughly.
2.Test paper incubation
The arrow on the test strip is facing downwards“human”Insert the diluted sample with the label facing upwards and incubate at room temperature.
3.result interpretation
10 Observation within minutes: The color development of the quality control line is effective, and a red band corresponding to the detection line indicates a positive type.
Product Information
brand |
Crystal Chem |
Product Name |
Human monoclonal antibody typing kit |
English name |
RapidYield Human Monoclonal Antibody Isotyping |
Item Number |
80617 |
Detecting targets |
Detecting targetsgG1/IgG2/IgG3/IgG4TheIgATheIgMKappa, lambda light chains |
Testing samples |
cell culture supernatant |
Detection Principle |
Nano particle lateral chromatography immunoassay with red bands for intuitive interpretation of results |
Product Specifications |
IgGSubclass detection strip (red)10 ArticleIgA/IgM/Light Chain Inspection Strip (Blue)10 Article, sample diluent4mL |
Storage conditions |
2-8℃ storage; The test strip can be stored at room temperature for a short period of time, and the diluent needs to be diluted2–8Refrigerate at ℃. |
Product application:
1.Hybridoma clone screening: Quickly identify positive clone types, accelerate monoclonal antibody strain screening and strain establishment.
2.High throughput typing of cell supernatant: Batch completion of anti-type identification on the culture list to improve experimental throughput.
3.Antibody engineering and expression validation: confirming the class of recombinant antibody expression products/Subclass and light chain correctness.
4.Basic research in immunology: Basic subtyping support for the study of antibody function, structure, and mechanism of action.
5.Pre development of antibody drugs: rapid typing of human monoclonal antibody candidate strains.
For more product details, please inquireCrystal ChemBeijing Bored Biotechnology Co., Ltd. is the general agent for the China region.