Operation steps:
Recommended method development process:
Excellent phospholipid removal effect:
Application ID: CTSLE01
*Attention:
(1) The recommended ratio of sample loading to diatomaceous earth filler is 1:1
(2) Dilute the high viscosity sample buffer before loading
(3) The recommended ratio of eluent to sample volume is 3:1, with multiple small amounts yielding better results
Comparison between SLE and LLE
Comparison of processing time
The time required for Cleanert SLE to process samples is significantly less than traditional LLE methods, and there is no need to remove the supernatant, which simplifies experimental operations and improves laboratory sample throughput.
Comparison of purification effects
Phospholipids are the main source of matrix effects. Compare Cleanert®The ability of SLE to remove phospholipid interference compared to traditional LLE shows that Cleanert SLE has a better effect on removing phospholipids.
Conclusion:
After purification by SLE method, the peak area of phospholipids was about 100 times lower than that by LLE method.
