Technical Background

The current basic research on cells mainly focuses on two-dimensional static culture, which differs greatly from the actual "dynamic+three-dimensional" mode, resulting in significant differences in cell morphology, cell differentiation, intercellular interactions, and in vivo dynamic environment. For example, changes in cell cytoskeleton reorganization, cell morphology, and gene protein expression. Therefore, we have developed a new in vitro cell culture system that enables researchers to analyze cell biology under different types of mechanical stimuli in a controllable and easily replicable manner. Using this system, we can gain a deeper understanding of how cells interact and grow under various forces. Its precise control and dynamic culture structure for cell growth and differentiation provide an ideal environment for cell development.

The cell tension system will bring cross disciplinary innovation

● More reliable evaluation before animal experiments● Stem cell differentiation mechanismThe correlation between mechanical stimulation force and cancer

● Research on the dynamic characteristics of biomedical materials and cells ● Rapid establishment of in vitro disease microenvironment

integratedCell Stretching Culture System

Touch screen operation interface

Preset loading waveform

HOW TO USE

1. Properly coat the culture chamber with matrix gel

2. Incubate the cells and let them grow on the bottom of the culture chamber, then place them in a stretching culture system

3. Connect the system to the power supply and press the power button. According to the experimental requirements, adjust the parameters using the touch screen panel

4. Monitor the state of cell stretching and replenish the culture medium in a timely manner

5. After the loading cycle ends, turn off the power. Evaluate the collected data, such as whether further optimization of stretching parameters is needed

6. Properly store the stretched cultured cells for subsequent experimental analysis

advantage

●Uniform Load - The culture chamber adopts pre embedded crossbar technology to ensure that each cell is uniformly subjected to strain along the tensile axis, with extremely low secondary loads in non axial directions

●High reproducibility - high-precision stepper motors ensure consistent motion programs in various speed and stretch ratio combinations, combining mechanical stability with the elasticity of the stretch film to ensure highly reproducible mechanical stimulation

●Integrated design - comes with a touch control screen, no need for a computer. Built in ARM chip, efficient and stable operation while being simple and easy to use

●Diverse modes - flexible configuration of different tension loading cycles, sizes, frequencies, durations, static hold, sine waveform, triangle waveform, rectangle, and various customized waveforms

●High throughput culture chamber - effective stretching area of 32mm × 32mm, PDMS material suitable for various laboratory analysis techniques, including cell fixation and fluorescence imaging

Equipment Parameters

other systems

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