Cell Stretching Instrument with Base Stiffness Control, Cell Base Hardness and Stiffness Can Control Tensile Stretching System

Can be within the range of substrate hardness, stiffness modulus 1-80kpaProvide axial and circumferential stress loading for various cultures of two-dimensional, three-dimensional cells and tissues.

Product maturity: Over 4000 articles have been published, and more than 150 successful units in China have used it.

Highlights:
1) The system provides axial and circumferential stress loading for various cultures of two-dimensional, three-dimensional cells and tissues; Not only does it have biaxial tensile force loading, but it also has uniaxial force application function
2) A computer-controlled stress loading system that provides controllable, reproducible, static, or periodic stress changes for cells cultured in vitro.
3) Use a vacuum pump to stretch the elastic silicone mold at the bottom of the culture plate and the bottom of the cell culture plateThe stretching degree can reach 33%, and the stress can be automatically adjusted and controlled through a gas device.
4) Based on flexible membrane substrate deformation and uniform stress distribution;
5) Real time observation of the response of cells and tissues under stress;
6) The flexstop isolation valve of * can make a portion of the cells in the same culture plate subjected to force, while a portion of the cells in the same culture well are not subjected to force, facilitating comparative experiments;
7) Integrated with pressure transmission instrument, it also has multi-channel cell pressure loading function;
8) Paired with Flex Flow parallel plate flow chamber, it can apply fluid shear stress while pulling cells;
9) Up to 4 channels, capable of running 4 different programs simultaneously, for conducting multiple comparative experiments on different tensile deformation rates;
10) Multiple frequencies, amplitudes, and waveforms can be run in the same program;
11) There are various types of simulated waveforms available for loading: static waveform, positive rotation waveform, cardiac waveform, triangular waveform, rectangular waveform, and various specially designed waveforms;
12) Better control of waveforms under ultra-low or ultra-high stress;
13) The computer system intelligently regulates the loading cycle, magnitude, frequency, and duration of tensile and tension forces
14) Loading and analyzing the biochemical reactions of various cells under tensile stress stimulation
15) Wide range of extension: 0-33%
16) Wide pulling frequency range: 0.01-5Hz

Flexercell's new product - CellSoft - Cell stress culture with different substrate hardness, cell tension and tension culture dish with different substrate hardness

●Range of elastic modulus1-80kPa

●Optional porous plate60mmand100mmculture plate

●BioFlex®CellSoftstandard6orifice plate

●Pulling cells on a flexible substrate

●Chamber glass slideCellSoft

●Surface protein coating, sterile individual packaging

CellSoftThere are many different types of culture plates, such as different hardness, different well plates, chamber slides (circular porous plates) used for microscopic observation, covalent coatingCollagenIOr other proteins that can provide static or dynamic tensile stress stimulation to cells. More importantly, the new typeCellSoftThe culture plate can be repeatedly digested with trypsin and inoculated with cells, and the protein coated surface can be reused up to three times.

Cover glass coated with microstructure gel

---Simultaneously controlling shape and stiffness to reproduce the internal environment

A micro structure polyacrylamide gel coated cover slide for cell culture is provided.
The gel based matrix comprises soft or rigid microstructures in the form of open microchannels (grooves) or pores.
Therefore, cells can be cultured on substrates that simulate the terrain features and stiffness of the in vivo environment.
>Available anytime
Directly inoculate cells
>Multiple types of rigidity
From very soft (1 kPa) to very hard (200 kPa)
>Extensive 3D design
Groove, square hole, circular hole, etc

Coated with fibronectin, therefore it can be used

Compatible with high-resolution optical microscope systems

Provided in a separate plastic bag in the form of water-based solution to maintain the characteristics of gel

Provide standard shapes: grooves and circular holes

Standard size: 24 mm circular cover glass (approximately 170 μ m thickness)

Compatible with infusion chamber

Typical arrangement of standard 24mm arc-shaped grooves and grooves:
Each cover slip has eight areas, characterized by varying widths (grooves) or diameters (circular holes): from 10 μ m to 100 μ m.
The depth of the structure is 10 μ m.
The following scheme describes the special case of circular wells, but the distribution is similar in the case of grooves

Additional Information:

The size of the cover glass can be modified as needed

The same type of gel can be adapted to culture dishes or porous plates

Other shapes of triangular holes, square holes, or rectangular holes can be manufactured as needed

Other depths can be manufactured according to requirements