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C3H/10T1/2Clone8 cell specific culture medium
NegotiableUpdate on 05/06
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Overview
C3H/10T1/2Clone8 cell specific culture medium C3H/10T1/2Clone8 cells are mouse embryonic fibroblast cell lines, and their culture medium generally contains components that support cell growth and differentiation, such as growth factors, vitamins, etc., which help maintain the normal morphology and function of cells, as well as their differentiation ability under specific conditions.
Product Details
C3H/10T1/2Clone8Cell specific culture mediumProduct Introduction
C3H/10T1/2 Clone 8The cell culture medium has been carefully optimized by the team, and after long-term testing, this product can maintainC3H/10T1/2 Clone 8Excellent growth state of cells.
This product already includesC3H/10T1/2 Clone 8The various components required for cell growth can be directly used without adding any ingredientsC3H/10T1/2 Clone 8Cell culture.
Item Number |
YS-XP9990 |
specification |
1*125ml/500ml |
classification |
Cell specific culture medium |
purpose |
For scientific research experiments only |
Main ingredients of the product:
MEMBasic medium 445 ml
Defined FBS 50 mL
P/S 5 mL
Transportation and storage
Transportation: Low temperature transportation in an insulated box containing biological ice packs
保存方法： 2℃～8℃, avoid light, store2Months;-20℃, avoid light, store6A month.
Cell culture:
C3H/10T1/2Clone8cellImproved from the culture medium, initially designed forC3H/10T1/2Clone8cellDesigned.C3H/10T1/2Clone8cellThe concentration of amino acids isMEMTwice as high, the vitamin concentration isMEMof4Double, use doubleHCO3-andCO2Concentration provides a better buffering effect. The glucose content in the initial formula was1000 mg/LLater, in order to meet the growth needs of certain cells, the glucose content was adjusted again4500 mg/LThis is what people often refer to as low sugar and high sugar.
C3H/10T1/2Clone8cellCulture medium is the preferred medium for adherent cell culture. According to the level of glucose content,C3H/10T1/2Clone8cellThe culture medium can generally be classified as high sugar type(below4500mg/L)And low sugar type(below1000mg/L)Two. Among them, high sugar typeC3H/10T1/2Clone8cellThe culture medium is conducive to the anchoring and growth of cells in a single location, suitable for tumor cells that grow rapidly but have difficulty attaching. When performing monoclonal antibody cell fusion, low sugar type is generally usedC3H/10T1/2Clone8cellCulture medium. High sugar typeC3H/10T1/2Clone8cellRapid cell growth can actually be detrimental to chromosome stability in fused cells.
Cell growth curve chart:
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Notes:
1ReceivedC3H/10T1/2Clone8cellPlease first check if the packaging is intact. If there is any damage, leakage, turbidity or other phenomena, please contact us in a timely manner. After the culture medium is frozen and thawed, a small amount of flocculent material may precipitate, which does not affect the normal use of the product.
2Please carefully read the product manual to understand the usage, storage method, expiration date, and other information of the product. If the product is contaminated due to operational errors or improper storage, the responsibility shall be borne by the customer.
3This product can only be used for scientific research. Some components in the culture medium have low harm to the human body. If in contact, rinse immediately with plenty of water.