C2C12 cell specific culture medium

C2C12Cell specific culture mediumProduct Introduction

C2C12The cell culture medium has been carefully optimized by the team, and after long-term testing, this product can maintainC2C12Excellent growth state of cells.

This product already includesC2C12The various components required for cell growth can be directly used without adding any ingredientsC2C12Cell culture.

Main ingredients of the product:

DMEMBasic medium445ml

Defined FBS 50 ml

P/S 5 ml

Transportation and storage

Transportation: Low temperature transportation in an insulated box containing biological ice packs

保存方法：2℃～8℃, avoid light, store2Months;-20℃, avoid light, store6A month.

Cell culture:

C2C12cellImproved from the culture medium, initially designed forC2C12cellDesigned.C2C12cellThe concentration of amino acids isMEMTwice as high, the vitamin concentration isMEMof4Double, use doubleHCO3-andCO2Concentration provides a better buffering effect. The glucose content in the initial formula was1000 mg/LLater, in order to meet the growth needs of certain cells, the glucose content was adjusted again4500 mg/LThis is what people often refer to as low sugar and high sugar.

C2C12cellCulture medium is the preferred medium for adherent cell culture. According to the level of glucose content,C2C12cellThe culture medium can generally be classified as high sugar type(below4500mg/L)And low sugar type(below1000mg/L)Two. Among them, high sugar typeC2C12cellThe culture medium is conducive to the anchoring and growth of cells in a single location, suitable for tumor cells that grow rapidly but have difficulty attaching. When performing monoclonal antibody cell fusion, low sugar type is generally usedC2C12cellCulture medium. High sugar typeC2C12cellRapid cell growth can actually be detrimental to chromosome stability in fused cells.

Cell growth curve chart:

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Notes:

1ReceivedC2C12cellPlease first check if the packaging is intact. If there is any damage, leakage, turbidity or other phenomena, please contact us in a timely manner. After the culture medium is frozen and thawed, a small amount of flocculent material may precipitate, which does not affect the normal use of the product.

2Please carefully read the product manual to understand the usage, storage method, expiration date, and other information of the product. If the product is contaminated due to operational errors or improper storage, the responsibility shall be borne by the customer.

3This product can only be used for scientific research. Some components in the culture medium have low harm to the human body. If in contact, rinse immediately with plenty of water.