Bio Beads S-X3 gel column

Bio Beads S-X3 gel column

Bio Beads S-X3, a polymer medium from Bole Corporation in the United States. The medium is neutral and porous polystyrene divinylbenzene microspheres, used for molecular weight exclusion chromatography of lipophilic polymers and organic elution solutes. The exclusion range of molecular weight from 400 to 14000 can be used to separate small molecular weight organic polymers and other hydrophobic substances. Such as insecticides, rodenticides, polycyclic aromatic compounds, and unsaturated lipids. The use of different eluents can affect the exclusion limit. useBioThe separation of Beads S-X medium requires a flowable eluent, therefore, the medium must be used inside the chromatography column. The stronger the aromaticity of the elution solvent, the higher the exclusion limit. The medium is compatible with reagents such as benzene, toluene, xylene, carbon tetrachloride, dimethylformamide, ketones, aromatic compounds, dichloromethane, o-dichlorobenzene, vinyl chloride, and trichlorobenzene.

BioBeads S-X3 gel columnMain applications:

1. Determination of multi-component residues of organochlorine pesticides in food GB/T 5009.19-2008 uses this medium to analyze pesticide residues.

2. It can be used to separate low molecular weight organic polymers and other hydrophobic substances, such as insecticides, rodenticides, polycyclic aromatic compounds, and unsaturated lipids.

3. Determination of Sudan 1, 2, 3 and 4 residues in food gel purification.

4. Testing of plasticizers in packaging materials.

BIO Bead S-X3 gel column

Specification: 400 * 25

Material: 316 stainless steel material

Packing: Bole BIO Bed Packing (* 200~400mesh)

Pharmacopoeia methods and related supporting equipment details

Method for determination of pesticide residues in the 2015 edition of the Chinese Pharmacopoeia
Preparation of test solution: Take the test sample, grind it into powder (pass through the third sieve), take about 1.5g, weigh it accurately, place it in a 50ml polystyrene stoppered centrifuge tube, vigorously shake and extract for 1 minute, then add a pre weighed mixture of 4g anhydrous magnesium sulfate and 5g sodium chloride powder, shake vigorously again for 1 minute, and centrifuge (4000 rpm) for 1 minute. Accurately suck 10ml of supernatant, decompress and concentrate to nearly dry at 40 ℃, transfer the mixed solution of cyclohexane ethyl acetate (-1:1) to 10ml volumetric flask in batches, add the mixed solution of cyclohexane ethyl acetate (1:1) to the scale, shake it well, transfer it to a centrifuge tube with 1g of anhydrous * added in advance, shake it, place it for 1h, centrifuge (filter it if necessary), take 5ml of supernatant, pass it through the gel permeation chromatographic column (400mm × 25mm, with BIO-Beads S-X3 packing inside; Using a mixture of cyclohexane and ethyl acetate (1:1) as the mobile phase; Purification was carried out at a flow rate of 5.0ml per minute. The eluent was collected for 18-30 minutes and concentrated under reduced pressure in a 40 ℃ water bath to near dryness. A small amount of n-hexane was added twice and dissolved in 1ml of n-hexane. The mixture was then transferred to a Florisil solid-phase extraction column [1000mg/6ml, pre washed with 10ml of n-hexane acetone (95:5) mixed solution and 10ml of n-hexane]. The residue was washed three times with n-hexane, each time 1ml. The washing solution was transferred to the same Florisil solid-phase extraction column and eluted with 10ml of n-hexane acetone (95:5) mixed solution. All elution solutions were collected and blown to near dryness on a nitrogen blower. Isooctane was added to a volume of 1ml and vortexed to dissolve. That's it.

Products required for sample preparation and purification of pesticide residue determination method in the 2015 edition of the Chinese Pharmacopoeia:

BIO Bead S-X3 gel column

400mm*25mm

BIO Ads S-X3 filler 200-400 mesh, 100g/bottle

PX-12A 12 bitdry-typeNitrogen blower

Florisil solid phase extraction column 1000mg/6ml, 30 tubes/box