Beijing Kilby Biotech 3D live cell automatic perfusion culture system

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

The Beijing Kilby Biological 3D live cell automatic perfusion culture system integrates the perfusion system into the culture chamber to achieve 3D cell perfusion culture. The system is used for the cultivation and expansion of organoids, providing solutions for organoid modeling, biological sample library construction, tumor organoid drug sensitivity testing, drug discovery, regenerative medicine, and other fields in a more standardized and automated manner.

Product Details

Beijing Kilby Biotech 3D live cell automatic perfusion culture systemSuitable for cell types:Including primary cells and induced pluripotent stem cells（iPSC）、 Organoids and cell lines, etcHealthy cells, diseased cells, and tumor cells can also be introduced

(1)Beijing Kilby Biotech 3D live cell automatic perfusion culture systemFunctional applications and device advantages

Utilizing the circulation of culture medium, simulating blood flow and low shear stress environment, combined with 3D culture to construct an in vitro model of cell like organs, which is closer to the in vivo environment of the human body. By introducing flow into the external environment, the physiological relevance of your research has been precisely improved,This provides an ideal tool for the research of 3D cellular organoids,Enabling you to generate more accurate models greatly increases confidence in the validity of the results. Thus, researchers can cultivate 3D cellular organoids more efficiently and reliably, accelerating the process of drug development and biomedical research.It is widely used in disease modeling, drug screening and toxicity testing, regenerative medicine and tissue engineering, developmental biology research, infection and immune research, personalized medicine, cancer research and other fields.

Significant benefits include:
uCompatible with multiple cell sources, including primary cells and induced pluripotent stem cells（iPSC）、 Organoids and cell lines, etcHealthy cells, diseased cells, and tumor cells can also be introduced
vAccelerate the differentiation and maturation of organoid cells, and enhance cell vitality
wDiversified cultivation methods with selectable gas-liquid interfaces, liquid-liquid interfaces, scaffolds, and flow schemes
xMeet the experimental requirements of multi cell/multi organ co culture, intercellular signal transmission, etc
yEquipped with optical windows on the top or bottom surface, imaging friendly and convenient for ideal real-time high-resolution imaging
zStrictly control multiple variables,Can simulate physiological characteristics such as blood circulation, interstitial fluid flow dynamics, etcIt can achieve complex model construction such as co culture of immune cells and vascularization; Used for studying various physiological processes, such as cell migration, differentiation, immune response, and cancer metastasis.
{Flexible and easy to use
|Save time and costs
}Long cell survival time, suitable for long-term cultivation

Instrument application and published articles:

Alaa Riezk, Alec O’Keeffe , Katrien Van Bocxlaer , VanessaYardley ,Simon L. Croft.Comparative assessment of macrophageresponses and antileishmanial efficacy indynamic vs. Static culture systems utilizingchitosan-based formulationsA series of diseases caused by protozoan parasites of the Leishmania genus, including visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). These parasites exist in the form of active pre flagellates in sand flies and proliferate in the form of flagellates in mammalian macrophages. The interaction between macrophages and parasites is crucial in leishmaniasis. The functions of macrophages, such as phagocytosis and macrophagy, are crucial for controlling parasitic infections.
The author studied the effects of dynamic and static culture systems on macrophage function and the efficacy of anti Leishmania drugs under simulated physiological fluid flow conditions. The study utilized chitosan based formulations and utilizedKirkstall Quasi Vivo Fluid FlowThe perfusion system was used to simulate physiological fluid flow, and the phagocytic and macrophagic effects of macrophages under dynamic and static conditions were compared, as well as the efficacy of anti Leishmania drugs.
The system is capable of directly observing the exposure of infected cells to different media perfusion rates and continuously monitoring the infection situation. This system allows multiple culture chambers to be connected in series and provides cell surface flow rates comparable to human interstitial fluid flow rates. Under static cultivation conditions, chitosan solution, blank chitosan TPE nanoparticles, and chitosan TPE nanoparticles loaded with AmB exhibited higher anti Leishmania activity than under dynamic cultivation conditions. Under low flow velocity (1.45 x 10 ^ -9 m/s) and high flow velocity (1.23 x 10 ^ -7 m/s) conditions, the anti Leishmania activity of these formulations was significantly reduced.The results emphasize the importance of considering fluid flow dynamics in in vitro studies, which is crucial for more accurate simulation of in vivo conditions. The dynamic culture system can better simulate the physiological conditions experienced by cells in vivo, thereby improving the correlation and reliability of experimental models.Under dynamic cultivation conditions, the anti Leishmania activity of chitosan based formulations and pure AmB was significantly reduced. This may be due to factors such as reduced drug accumulation caused by fluid flow and decreased macrophage function (such as phagocytosis and macrophagocytosis).