Beijing Kilby Animal Embryo Rotation Culture System

Main application scenario: RCCS embryo in vitro cultivation system produced by Beijing Kilby Biotechnology Co., LtdAnimal Embryo Rotation Culture System RCCS
Mainly used for dynamic 3D, dynamic suspension, organoid culture, etc; It can also be used for anaerobic, oxygen rich and other environmental cultures, early in vitro embryo development cultures, and culture scenarios of cells or tissues such as cartilage, myocardium, blood vessels under certain pressure environments; Widely used in embryonic stem cell research, developmental biology research, genetic research, regenerative medicine and other fields.

Simulate microgravity（Simulated microgravity (s - μ g) can indeed accelerate the aggregation of alpha synuclein (α - syn) and induce oxidative stress, which has been validated in an in vitro Parkinson's disease (PD) model. Research has found that under the environment of s - μ g, the levels of monomers and aggregates of α - syn significantly increase, accompanied by an increase in the level of oxidative stress marker malondialdehyde (MDA), indicating an exacerbation of lipid peroxidation. In addition, the aggregation tendency of α - syn shifts from monomeric forms to more complex aggregate forms, which may further exacerbate neuronal damage.

Oxidative stressPlays an important role in the pathological mechanism of PD. Alpha syn oligomers induce ROS (reactive oxygen species) production through a metal ion dependent mechanism, leading to cellular toxicity. Under simulated microgravity conditions, the activity of intracellular antioxidant enzymes (such as superoxide dismutase, SOD) failed to counteract the increase in ROS, resulting in a significant increase in intracellular oxidative stress levels. This oxidative stress not only affects cellular metabolism, but may also trigger apoptotic pathways.

Main performance of animal embryo rotation culture system RCCS
Reproductive and developmental toxicity evaluation:The animal embryo rotation culture system has important application value in the evaluation of drug reproductive and developmental toxicity. It can simulate the internal environment, allowing the embryo to receive the effects of drugs or chemicals without being influenced by the mother, directly observing and studying their impact on embryo development, such as deformities, growth retardation, and other issues, thus providing a basis for the safety evaluation of drugs. For example, using the WEC model of rats and rabbits for joint research can more comprehensively and quickly predict the reproductive and developmental toxicity risks of the test substance

Research on the mechanism of embryonic development:This system provides a powerful tool for studying the normal growth and development process of embryos and the underlying signaling pathways. By precisely controlling the culture conditions in vitro, such as oxygen concentration, carbon dioxide concentration, temperature, etc., it is possible to deeply observe and analyze the morphological changes, cell proliferation and differentiation, gene expression, etc. of embryos at different developmental stages, thereby revealing the molecular mechanisms and regulatory factors of embryonic development

Research on fertilized egg transplantation:In the field of assisted reproductive technology, animal embryo rotation culture systems can be used for in vitro culture and evaluation before fertilized egg transfer. Being able to provide a stable and suitable growth environment for the fertilized egg, allowing it to develop normally in vitro before transplantation, improving the quality of the fertilized egg and the implantation rate after transplantation, thereby increasing the success rate of pregnancy

Stem cell and organoid culture:In addition to animal embryos, the system can also support stable development of stem cells and organoids in a dynamic rotating microenvironment. By simulating the physiological environment inside the body, it promotes the proliferation, differentiation, and organoid formation of stem cells, providing important technical support and model systems for stem cell therapy and organ regeneration medicine research