BIOCOLOR sulfated glycosaminoglycan (sGAG) detection kit

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kit Blyscan™Sulfated glycosaminoglycan(sGAG)Detection is a quantitative dye binding method used to analyze sulfated proteoglycans and glycosaminoglycans. The following are the main details of the product:

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kitScope of application

• This test can measure the total amount in the samplesGAGContent and ability to distinguishN-Sulfation andO-Sulfated glycosaminoglycans.

• Suitable for in vivo tissues (such as cartilage, soft tissue), in vitro cell culture (cells, matrix, culture medium), synovial fluid, urine, and other soluble samples.

B1000Sulfated glycosaminoglycan(sGAG)detectionMain components of the reagent kit

1. Dye reagentUsed for specific labeling of sulfated polysaccharides.

2. Dissociation reagentSodium salts containing anionic surfactants can enhance the absorption properties of dyes.

3. reference standard

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kitMain Features

• detection limit：0.25 µg/100 µl (2.5 µg/ml).

• time requiredApproximately required for each test1 Hour.

• Storage conditionsAll components are15-25ºCStable storage for one year under certain conditions;sGAGAfter opening, standard products should be stored in+4ºC.

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kitTesting steps

1. Sample preparationThe sample must be free of particulate matter and soluble in the detection buffer.

2. Detection settingsAdd to the sampleBlyscanDye reagents, mixed and incubated30 minute

3. centrifugation: Separation by centrifugationsGAG-Dye complex.

4. measurementDissolve the bound dye in the dissociation reagent and transfer it to a microplate656 nmMeasure the absorbance at the location.

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kitApplication and Limitations

• Suitable for a wide range of mammalian proteoglycans, including keratin sulfate, dermatan sulfate, and heparan sulfate.

• Not applicable to small sulfated disaccharide fragments or samples containing alginates.

B1000Sulfated glycosaminoglycan(sGAG)detectiontest kitTo measure various biological samplessGAGThe level provides an efficient method for research related to connective tissue and extracellular matrix.

B1000Sulfated glycosaminoglycan(sGAG)detectionReagent kit ordering:

B1000 Blyscan sGAG Assay, (110 assays), [Standard Kit Size]

B3000 Blyscan sGAG Assay, (440 assays), [Large Economy Kit]