BIOCOLOR S2000 Insoluble Collagen Protein Detection Kit

Sircol® Insoluble Collagen Assay (S2000) -insolubleQuantitative detection solution for collagen protein

Product Introduction

Sircol® Insoluble Collagen Assay (S2000)It is a staining detection tool designed specifically for quantitative detection of insoluble collagen, widely used in biomedical research. This detection kit can quickly and accurately determine the content of insoluble collagen in tissue samples, and is suitable for various in vivo and in vitro mammalian samples.

SircolInsoluble collagen detection kitS2000Product Advantages

• Fast and efficientThe entire testing process only requires about4Hours, easy to operate, suitable for various laboratory conditions.

• high sensitivityThe detection limit is10 µg/100µlSuitable for the detection needs of trace samples.

• Gentle treatmentAdopting mild acidity and temperature treatment, it does not require strong acid or high temperature treatment compared to traditional methods.

• widely usedCan be applied to evaluate newly generated collagen fibers during tissue growth, repair, and remodeling.

• convenient and practicalAll required reagents and consumables are included in the reagent kit, providing a one-stop solution for experimental needs.

SircolInsoluble collagen detection kitS2000Scope of application

Sircol® Insoluble Collagen AssaySuitable for:

• Organizational growth and developmentresearch on

• Organizational repair and wound healingThe evaluation

• Disease Model ResearchMonitoring of collagen changes in

• Biomaterials and Regenerative Medicine研究

SircolInsoluble collagen detection kitS2000productcomponent

• Fragmentation ReagentUsed for processing non soluble raw fibers, containing diluted acetic acid, antibacterial agents, and surfactants.

• Dye ReagentA dye reagent designed specifically for binding to collagen under detection conditions.

• Alkali Reagent: Used for extracting collagen-Recovering dyes from dye complexes.

• reference standardContains soluble denatured collagen.

SircolInsoluble collagen detection kitS2000Testing steps

1. Sample processing: Will20-30 mgWet tissue samples or non sol residue are placed in2 mlAdd the corresponding volume into the digestive tractFragmentation Reagent, placed in65°Cincubation2-3Hour.

2. centrifugationAfter incubation, centrifuge and collect the supernatant for analysis.

3. Staining reactionAdd to the sampleSircolDye reagents bind collagen with dyes to form precipitates.

4. Washing and releasing dyesUse acid salt washing reagent to remove unbound dye, then add alkaline reagent to release the dye for measurement.

5. Data and AnalysisMeasure the absorbance using colorimetric method and calculate the concentration of insoluble collagen based on the standard curve.

SircolInsoluble collagen detection kitS2000How to place an order?

S2000 Sircol INSOLUBLE Collagen Assay,(100 assays)

S6000 Sircol INSOLUBLE Collagen, (440 assays)

S4000 Sircol S1000 x 1 plus S2000 x 1 Combination pack

SFRAG S2000 Fragmentation Reagent, (3 x 110 ml)

S2010 S2000 Denatured Collagen Standard 1mg/ml, (3 x 5ml)