BIOCOLOR Elastic Protein Detection Kit General Agent

F2000 Fastin ™ Elastin assay kit

The F2000 elastin detection kit is designed specifically for tissue engineering and elastin research, providing a highly sensitive and easy-to-use detection solution suitable for scientific research.

summary:BIOCOLOR Elastic Protein Detection Kit General AgentFastin ™ Elastin assay is a quantitative dye binding method designed specifically for analyzing elastin extracted from mammalian tissues and cultured cells. It is suitable for research purposes and can accurately measure various forms of elastin, including soluble pre elastin, lactiflorin elastin, and insoluble elastin after dissolution.

Main features of elastin detection kit

Quantitative analysis: Measure the elastin content in tissue extracts and cultured cells.

Detection limit: Low Z can detect elastin at a concentration of 5 μ g/100 μ gl.

Multi purpose application: Suitable for evaluating the elastin content in various biological samples, including animal tissues and cells cultured in vitro.

Reliable and reproducible: Use dye reagents that specifically bind to the amino acid sequence of elastin to ensure accurate results.

The main components of the elastin detection kit are:

1. Fastin dye reagent: in citric acid phosphate buffer containing TPPS (tetraphenyl-21H, 23H porphyrin tetrasulfonate).

2. Elastic protein precipitation reagent: including trichloroethylene SUAN and hydrochloric acid.

3. Elastin standard: High molecular weight alpha elastin.

4. Dye dissociation reagent: composed of guanidine hydrochloride and propanol.

5. Oxalic acid: used for sample preparation and elastin extraction.

Experimental steps for elastin detection kit:

1. Sample preparation: Treat tissue samples with oxalic acid at 95-100 ° C to convert insoluble elastin into soluble form (alpha elastin).

2. Measurement process: Form an elastin dye complex, remove unbound dye, then release bound dye and measure using a microplate reader.

3. Measurement: Obtain results by comparing the absorbance of the sample with the standard curve.

Application scope of elastin detection kit

Organizational analysis: suitable for quantifying elastin in tissues such as skin, heart, liver, and lungs.

Cell culture research: Measure the elastin production of fibroblasts and other cell types in vitro culture.

How to order an elastin detection kit?

Standard reagent kit (product code: F2000): Contains the reagents required for 110 measurements.

Economic package (product code: F4000): Includes reagents required for 440 measurements.

How to store the elastin detection kit?

● Store at room temperature. Do not freeze to avoid solubility issues.

After opening the reagent kit, store the dye reagent and elastin standard at+4 ° C to maintain stability.

Instruments and requirements for elastin detection kit

A microplate reader equipped with a 513 nm absorption peak filter is required.

Mechanical oscillator and centrifuge, with a centrifugal force of 13000 x g.

Reference list for elastin detection kit: Over 1000 references, multiple published in Nature and Science journals

Ahn, G., Min, K., Kim, C., Lee, J., Kang, D., reports, J. W.-S., & 2017 Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules.

Bengtsson, E., Hultman, K., Dunér, P., Reports, G. A.-S., & 2017 ADAMTS-7 is associated with a high-risk plaque phenotype in human atherosclerosis.

Godinho, M., Thorpe, C., reports, S. G.-S., & 2017 Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing.

Kitano, K., Schwartz, D., Zhou, H., … S. G.-N., & 2017 Bioengineering of functional human induced pluripotent stem cell-derived intestinal grafts.

Simões, I., Vale, P., Soker, S., Atala, A., Reports, D. K.-S., & 2017 Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications.

Chen, W., Wang, Z., & Missinato, M. (2016). Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration. Science.

Where can I purchase an elastin detection kit?

BIOCOLOR Elastic Protein Detection Kit General AgentCompany: Beijing Qunxiao Keyuan Biotechnology Co., Ltd