BGC-823 human gastric adenocarcinoma poorly differentiated cells

1、 Overview of BGC-823 Cell Products

BGC-823 human gastric adenocarcinoma poorly differentiated cellsIt is a classic cell line widely used in biomedical research. This cell is derived from undifferentiated adenocarcinoma of gastric cancer in a 62 year old Chinese individual, exhibiting epithelial like growth characteristics and proliferating in an adherent manner under in vitro culture conditions. The doubling time of BGC-823 cells is about 24 hours, which is suitable for conducting various tumor biology research and drug screening experiments.

2、 Basic information of BGC-823 cells

3、 Characteristics and biological properties of BGC-823 cells

BGC-823 cells have distinct biological characteristics: CEA positive, poorly differentiated gastric cancer. These unique molecular markers and biological behaviors make them important tools for researchers to conduct research on tumor mechanisms and drug development.

During the cultivation process, the cells exhibited stable proliferation ability and good experimental reproducibility, which can meet various in vitro experimental requirements. It is recommended to use STR identification to verify cell identity and ensure the reliability of experimental data.

4、 Cultivation conditions and operational suggestions

Culture medium configuration:RPMI-1640 + 10% FBS + 1% P/S。 The cultivation conditions are 37 ℃, 5% CO ₂, and saturated humidity. It is recommended to replace the fresh culture medium every 2-3 days, with a passage ratio of 1:2 to 1:4.

Cell revival:Quickly thaw the cryovial in a 37 ℃ water bath, centrifuge and resuspend in wan quan medium, and transfer to a culture bottle for cultivation.

Cell cryopreservation:Use a cryopreservation solution of 55% basic medium+40% FBS+5% DMSO and store in liquid nitrogen for a long time.

5、 Application Fields

BGC-823 cells are widely used in the following research fields:

• Research on poorly differentiated gastric cancer, screening of therapeutic drugs, and evaluation of traditional Chinese medicine's anti gastric cancer activity

• Sensitivity testing of anti-tumor drugs and optimization of treatment plans

• Screening and validation of tumor molecular markers

• Research on the Mechanism of Cell Signal Transduction Pathway

• Research on Tumor Microenvironment and Immune Escape

6、 Precautions

1. Cell culture must be carried out in a biosafety cabinet, strictly following sterile operating procedures.

The quality of culture medium and serum has a significant impact on cell growth, and it is recommended to use high-quality serum.

3. The cell state is affected by environmental factors such as temperature and pH, and regular observation of cell morphology and growth status is necessary.

4. This product is for scientific research purposes only and cannot be used for clinical diagnosis or treatment.

5. After receiving the cells, please conduct STR identification in a timely manner to confirm the identity of the cells.

7、 Product specifications and ordering information

If you need to learn more about BGC-823 cell products or obtain technical support, please feel free to contact our professional customer service team. We will provide you with free cell culture technical guidance throughout the process to ensure the smooth progress of your scientific research work.

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