Animal tissue/cell total RNA rapid extraction kit

Animal tissue/cell total RNA rapid extraction kit

1. Product Description: How to Translate

Xinli Zhihe®Animal tissue/cell total RNA rapid extraction kitBased on silica gel column purification technology, it can be used for high-quality extraction of total RNA from animal tissues and cells. The extraction process does not require the use of toxic organic solvents such as phenol and chloroform, and the operation is simple. The extracted RNA product has high purity and minimal residual impurities such as proteins and gDNA.

2. Product Features:

(1) Easy to operate, simple and fast, the experimental operation can be completed within 8 minutes.

(2) The sample has a wide range of applications.

(3) The extracted RNA fragments are large, of high purity, and of good quality.

Instructions for Use

1. Product components:

2. Storage and transportation conditions:

It can be stored for 12 months at room temperature (15-30 ℃). At room temperature（15~30℃）Transportation.

3. Notes:

(1) Before use, please add an appropriate amount of anhydrous ethanol to the protein removal solution RW1 and rinse solution RW2 as indicated on the label.

(2) Before use, please check if there is any precipitation in the lysis buffer RAL and protein removal solution RW1. If necessary, wait for the precipitation to dissolve in a 37 ℃ water bath before continuing to use.

(3) To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

(4) When using this reagent kit, please wear lab clothes, disposable latex gloves, disposable masks, and use RNase Free consumables to avoid RNase contamination.

(5) All experimental operations should be carried out at room temperature of 15-25 ℃ unless otherwise specified.

relevant data

1. Hela cell RNA extraction efficiency test:

The RNA of Hela cells was extracted by this product and the competitive product respectively. The extract was detected by gel electrophoresis. From the band brightness, it can be seen that the extraction effect of this product is better than that of the competitive product.

2. Testing of RNA extraction efficiency from pig liver tissue:

The RNA of pig liver tissue was extracted by this product and the competitive product respectively. The extract was detected by gel electrophoresis. From the brightness and width of the strip, it can be seen that the extraction effect of this product is better than that of the competitive product.

3. Determination of RNA extraction concentration:

RNA was extracted from samples such as pig liver and kidney using this product and its competitors, and the concentration of RNA extraction was detected. The results are shown in Figure 1 and Figure 2. The quality and concentration of RNA extraction from our product are superior to those of our competitors.

Frequently Asked Questions

1. Low RNA yield?

Root cause analysis:The extracted organization is not fresh;Improper sample storage or repeated freeze-thaw cycles.

Solution:Adopting fresh organization；Organize homogenate on ice to avoid high temperature induced RNA degradation;Sample placed at -80Store at ℃, it is recommended to pack according to the quantity to avoid repeated freezing and thawing.

2. Low RNA purity?

Root cause analysis:Electrophoretic contamination or electrophoresis environment;Ethanol residue; Residual salt ions.

Solution:After cleaning the electrophoresis tank with DEPC water several times, electrophoresis was performed using RNas during the operation processe-FREE consumables;Ensure stepsThe centrifugation time and centrifuge speed meet the requirements of the instruction manual;ensureRinse solution RW2Wash twice and avoid the adsorption column from touching the centrifuged liquid after centrifugation.