ABI7500 Real time Fluorescence Quantitative PCR Instrument Shanghai Huicheng Spot

ABI7500 Real time Fluorescence Quantitative PCR Instrument Shanghai Huicheng Spotprovide， The 7500 Real Time PCR Instrument is a real-time nucleic acid amplification and detection instrument that uses a dual standard hydrolysis probe fluorescence detection method to measure the nucleic acid signal of reverse transcribed DNA or RNA and convert it into comparable quantitative readings.

The ABI7500 rapid real-time quantitative PCR instrument is an integrated platform for specific target gene detection and quantification. 7500 combines PCR thermal cycling, fluorescence detection, and various application analysis software to dynamically observe the gradual increase of PCR amplification products in each reaction tube during each PCR cycle. Quantitative results can be obtained immediately after the PCR experiment, without gel electrophoresis analysis, purification of PCR products and any experimental operation. The 7500 real-time fluorescence quantitative PCR instrument uses a 96 well reaction plate or a single or 8-tube, with a reaction volume of 25-100 microliters. The experiment can be completed in less than 2 hours. Compared with other artificial gene quantitative analysis methods such as Northern blotting or RNase protection assays, the 7500 real-time fluorescence quantitative PCR system has the advantages of time-saving, high sensitivity, good accuracy, and wide linear range. The 7500 real-time fluorescence quantitative PCR system was newly launched by Applied Biosystems, a global real-time quantitative PCR technology company, in January 2004. It has excellent quality, accurate and reliable experimental results, and an extremely high cost performance ratioExcellent.
ABI7500 Real time Fluorescence Quantitative PCR Instrument Shanghai Huicheng Spotprovide

The main applications of real-time quantitative PCR are:
Real time quantitative PCR is widely used, including gene expression analysis based on relative quantitative analysis, pathogen absolute quantitative analysis based on standard curves, qualitative SNP genotype analysis of nucleic acid sequences after PCR amplification, and positive/negative result determination based on positive internal controls
Rapid thermal cycle
The standard mode of the 7500 real-time fluorescence quantitative PCR system uses a reaction volume of 25-100 microliters and can be tested using a 96 well plate or a single tube/8-tube combination. In addition, the 7500 can also be upgraded to a fast thermal cycling sample base, which is an optional component installed by AB company engineers. The rapid thermal cycling sample base adopts a new 96 well reaction plate, which has a small space above the liquid in the tube and can support experiments with a reaction volume of 5-30 microliters. Using the upgraded 7500 sample base, a new 96 well reaction plate, and a new rapid thermal cycling quantitative PCR premix reagent, real-time quantitative PCR experiments can be completed within 40 minutes, and the experimental results are consistent with the standard 2-hour mode.