685975-902 Agilent liquid chromatography column

685975-902Agilent liquid chromatography column

It is a high-performance reverse phase chromatography column designed specifically for high-throughput, high separation liquid chromatography analysis, suitable for conventional useHPLCCompared with ultra-high performance liquid chromatography（UHPLC）System. Its core advantage lies in its ability to‌2.7 µmparticle size‌Realize proximity to Asia2 µmThe separation performance of the packing material, while significantly reducing the system back pressure, is compatible with traditional instrument platforms.

brand：Agilent

series‌：InfinityLab Poroshell 120

‌model‌：685975-902

‌Type of phase‌：SB-C18(Spatial hindrance)C18Fixed phase)

‌Inner diameter（ID）‌：‌4.6 mm‌

‌length‌：‌100 mm‌

‌particle size‌：‌2.7 µm‌

‌aperture‌：120.

‌Filler type‌Surface porous particles（Superficially Porous Particle, SPP）

‌Carbon carrying capacity‌：‌7.5%‌

‌end-capping‌: No

‌pHscope‌：‌1–8‌At lowpHExcellent performance under conditions

‌temperature tolerancescope‌：‌90 °C‌（pH 1–6）The‌40 °C‌（pH 6–8）

‌rated pressure‌：‌600 bar‌

‌Supported Platforms‌：HPLC / UHPLC

‌Detached Mode‌Reverse phase chromatography

‌Protection column‌: None (requires separate selection)

‌Applicable range of molecular weight‌：0–3000 Da

performance characteristics

‌High chemical stability‌: Especially suitable for‌Strong acidic mobile phase‌(If containing lowpHBuffer solution), inpH 1–8Long term use within the scope is not easily degradable.

‌Excellent peak shape and resolution‌The surface porous structure provides faster mass transfer rate, improves column efficiency and separation speed, and is suitable for rapid analysis of complex samples.

‌Long-Life Technology‌：Poroshell 120The technology significantly extends the lifespan of the column and reduces the cost of method development and daily testing.

‌Wide applicability‌Suitable for the analysis of various compounds such as small molecules, peptides, metabolites, etc., especially suitable forLC-MSBefore useHPLCSeparate.

Application scenarios

Drug impurity analysis

Metabolite detection in biological samples

Peptide and protein purification pretreatment

Method development and method transfer (compatible with traditional methods)HPLCSystem)

Usage suggestions and precautions‌

‌Mobile phase recommendation‌: Acetonitrile/Water or methanol/Water system, can be added0.1%Formic acid enhances peak shape, especially suitable for acidic compounds.

‌balance time‌When using or replacing the mobile phase, it is recommended to use10–20Equilibrium of mobile phase with double column volume.

‌Save solvent‌When not in use for a long time, it is recommended to use‌acetonitrile:water= 80:20‌Save and avoid the growth of microorganisms in the aqueous phase.

‌avoid‌: Strong alkaline conditions（pH > 8）Long term rinsing with pure water and insufficient dilution of high salt concentration buffer solution.