5020-89921 GL liquid chromatography column

5020-89921 GL liquid chromatography column

It is a reverse phase liquid chromatography column optimized for the analysis of highly polar compounds, belonging to the InertSustain AQ-C18 series jointly developed by Shimadzu and GL Sciences. It is widely used for the detection of difficult to retain polar substances such as drug metabolites, amino acids, nucleotides, and organic acids.

This series of chromatography columns uses a new high-purity spherical silica gel (ES silica gel) as the matrix, and precisely controls the bonding density and spacing of C18 long-chain through unique technology, effectively preventing "hydrophobic collapse" under high water phase or pure water mobile phase conditions, thereby significantly improving the retention ability of polar compounds.

Item number: 5020-89921

Particle size: 3 μ m

Inner diameter: 2.1 mm

Column length: 50 mm

Fixed phase: InertSustain AQ-C18 (octadecyl bonding)

Specific surface area: 350 m ²/g

Aperture: 100 Å (10 nm)

End group sealing: Yes

Pressure resistance upper limit: 50 MPa (HP series)

Matrix: High purity spherical silica gel (new ES silica gel)

PH range: 1-10

Carbon content: 13%

USP Number: L1

Applicable system: UHPLC/HPLC, especially suitable for the development of analytical methods under high water phase mobile phase conditions

This model is widely used in the fields of environmental protection, food, pharmaceuticals, etc. for the analysis of highly polar substances such as organic acids and nucleotides.

Core advantages:

1. Stronger retention of polar compounds: They can maintain stable retention even under 100% aqueous conditions, making them suitable for the separation of early peak components in gradient elution.

2. Good peak symmetry: Thanks to high inert fillers and sufficient end group sealing, sharp and symmetrical peak shapes can be obtained for alkaline, acidic, and metal coordination compounds, reducing tailing.

3. Wide pH tolerance: It can operate stably within the pH range of 1-10 and is suitable for the analysis needs of various complex samples.

4. High column efficiency and reproducibility: Excellent inter batch consistency, suitable for method development and validation, meeting GMP/GLP laboratory requirements.

5. Compatible with mass spectrometry detection: Due to the high proportion of organic phase during elution, the ionization efficiency is improved, making it particularly suitable for mass spectrometry techniques such as ESI-MS to enhance detection sensitivity.

Reference for practical application cases

This chromatographic column exhibits excellent performance in the following analyses:

Vitamin B testing: Polar vitamins such as pyridoxine can still be retained under low organic phase conditions, achieving baseline separation.

Metabolite analysis in biological matrices, such as amino acids and organic acids in plasma and urine, benefits from high inert fillers to reduce non-specific adsorption.

Analysis of drug impurities: For example, the determination of relevant substances in pyrazine tablets can achieve a separation degree of>2.0, meeting the requirements of the pharmacopoeia.