Alma blue cell proliferation and toxicity detection reagent

NegotiableUpdate on 05/06

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Overview

Alamar Blue Cell Proliferation and Toxicity Detection Reagent $r $n The working principle of Alamar Blue is that it is a redox (REDOX) indicator that changes color based on the metabolic reduction of cells. The oxidized state of Lingtianqing appears purple blue and has almost no fluorescence. Its reduced state product, resorufin, turns pink and highly fluorescent, and the fluorescence intensity produced is proportional to the number of respiratory cells. Alma blue is used as a direct indicator for quantitatively detecting cell viability and toxicity by detecting oxidation levels during respiration.

Product Details

Alma blue cell proliferation and toxicity detection reagent

Product Description
description	Alamar Blue is a cell viability assay reagent that contains an indicator with cell membrane permeability, non toxicity, and weak blue fluorescence, namely resazurin. Since 1993, Lingtianqing has been cited as a reliable and trustworthy reagent. Alma blue is an effective and non-toxic substitute for MTT (thiazole blue). Alma blue can quantitatively detect the proliferation of human, mammalian, bacterial, fungal, and mycoplasma cells, and can also be used for cytokine bioactivity, cell viability analysis, in vitro cytotoxicity determination, and cell growth monitoring. The working principle of Almablue is that it is a redox (REDOX) indicator that changes color based on cellular metabolism and reduction. The oxidized state of Lingtianqing appears purple blue and has almost no fluorescence. Its reduced state product, resorufin, turns pink and highly fluorescent, and the fluorescence intensity produced is proportional to the number of respiratory cells. Alma blue is used as a direct indicator for quantitatively detecting cell viability and toxicity by detecting oxidation levels during respiration. The color change of Alma Blue can be detected by a regular spectrophotometer, with a detection wavelength of 570nm and a reference wavelength of 600nm. The fluorescence change of Alma Blue can be detected by a fluorescence spectrophotometer, with an excitation wavelength between 530-560nm and an emission wavelength of 590nm; Compared with analytical methods such as trypan blue, TTC, MTT, MTS, etc., Alma blue is a single reagent that can continuously and rapidly detect cell proliferation. Alma blue is non-toxic and harmless to cells, does not interfere with the electron transport chain, does not interfere with cell respiration or function, and does not affect the activity of cell antibody synthesis and secretion. Therefore, it is suitable for continuous observation and further observation of the proliferation of the same batch of cells, with the characteristics of easy operation and almost no interference with normal metabolism.
appearance	solution
technical indicators	5mL（500T） 10mL（1000T） 50mL（5000T）
Product Usage	Cell proliferation and toxicity testing
Usage	Please refer to the product manual for details
Precautions	1. Alamar Blue in its reduced state is highly unstable in water or water-soluble buffer solutions such as PBS, but very stable in the culture medium. To determine the absorbance/fluorescence values of specific experimental reduced state Alamar Blue, it is necessary to prepare 100% reduced state Alamar Blue reagent: mix Alamar Blue with cell culture medium in a ratio of 1:10, and sterilize under high pressure for 15 minutes. 2. Appropriate cell density can increase detection sensitivity. For a 96 well plate, it is recommended to inoculate 100 μ l of cells per well, with a cell concentration range of 100-10000 adherent cells per well and 2000-50000 suspended cells per well, using the culture medium as a blank control. For the 384 well plate, both cell concentration and inoculation volume were halved. 3. The two major variables that affect cell response to Alamar Blue are incubation time and plate density. It is recommended to explore and optimize these two factors before conducting the experiment. Excessive cell density or prolonged incubation time can lead to secondary reduction reactions, where the red product stops increasing and begins to decay, resulting in a significant decrease in absorbance/fluorescence levels accompanied by the disappearance of red. 4. Microbial contamination can reduce Alamar Blue. Therefore, using Alamar Blue detection on contaminated cells can result in incorrect results. 5. Alamar Blue can be detected using a spectrophotometer or fluorometer, but it has high fluorescence sensitivity and small experimental error. Fluorescence detection is recommended. 6. For your safety and health, please wear lab coats and disposable gloves when operating.
Basic Information
Storage/Preservation Methods	Store at 4 ℃ away from light, with a shelf life of 12 months.

Alma blue cell proliferation and toxicity detection reagentKind reminder: This product is only for scientific research experiments and does not support clinical research