Fluorescent probe Ub-Rh110MP

Chinese Introduction:

UbiqBioFluorescent probe Ub-Rh110MP：Ub-Rh110MP (UbiQ-126) is a quenched fluorescent substrate for deubiquitinases (DUBs). The cleavage of the amide bond between the Gly76 and Rhodamine 110 moieties of ubiquitin releases highly fluorescent Rh110 morpholincarbonyl groups, which exhibit higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 was prepared through full chemical synthesis.

English Introduction:

UbiqBioFluorescent probe Ub-Rh110MP：Ub-Rh110MP (UbiQ-126) is a quenched, fluorescent substrate for deubiquitylases (DUBs). Cleavage of the amide bond between Gly76 of ubiquitin and the Rhodamine110 moiety releases the highly fluorescent Rh110-morpholinecarbonyl which exhibits a higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 is prepared by total chemical synthesis.

Product cited literature:

1.Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007). ncbi.nlm.nih.gov/pubmed/17869210

2.El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010). ncbi.nlm.nih.gov/pubmed/21117055

3.Lavis, L.D., et al. Fluorogenic label for biomolecular imaging. ACS Chem. Biol. 1, 252-260 (2006). ncbi.nlm.nih.gov/pubmed/17163679

4.Terentyeva, T.G., et al. Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters. Bioconj. Chem. 22, 1932-1938 (2011). ncbi.nlm.nih.gov/pubmed/21905728