- Phone
-
Address
Room 408-410, Building 2, Hengchuang Technology Park, 128 Jinling East Road, Weiting, Suzhou Industrial Park
Product Categories
Suzhou Ark Biotechnology Co., Ltd
(Experimental Service) Western Blot
NegotiableUpdate on 05/06
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
(Experimental service) Western Blot refers to the separation of protein samples by polyacrylamide gel electrophoresis, and then transfer them to a solid carrier (such as nitrocellulose film), and then use antibodies to detect the target protein through immunological reaction, and analyze the degree of gene expression. Can be denatured with alkali to prevent hydrolysis. Denaturation with formaldehyde.
Product Details
(Experimental Service) Western BlotIt is a molecular biology technique widely used for detecting the expression and quantification of specific proteins. The core process is divided into three steps: first, protein samples are separated by molecular weight through SDS-PAGE gel electrophoresis; Then transfer the separated protein bands onto PVDF or NC membranes; Then, immunological testing is performed by blocking non-specific sites with blocking solution, followed by incubating specific primary antibodies and enzyme labeled secondary antibodies targeting the target protein in sequence. Finally, a chemiluminescence substrate is added for reaction, and the signal is captured by an imaging system.
(Experimental Service) Western BlotIt has high specificity and sensitivity, and is the core method for verifying gene expression, protein modification, and comparing protein level differences between different samples. This method is widely used in the fields of molecular biology, biochemistry, and immunogenetics.
Similar Product Recommend