Bole electrophoresis three piece transfer printing system 2026 price

Bole electrophoresis three piece transfer printing system 2026 price

In Western Blot experiments, electrophoretic separation and protein transfer are the two core processes that determine the final strip quality. However, many laboratories have long faced the problem of equipment fragmentation in these two links. The electrophoresis tank uses brand A, the transfer tank uses brand B, and the power supply is brand C. The interface is incompatible, the parameters are not matched, and the operation process is fragmented, which not only prolongs the experimental cycle but also increases the probability of operational errors.

The Bio Rad 1658033 package is an integrated solution designed to address this pain point. It integrates a small vertical electrophoresis tank (1658001), electrophoresis transfer core module (1703935), and basic power supply (1645050) on a unified hardware platform, covering the core steps from SDS-PAGE protein separation to Western Blot wet transfer. Users do not need to purchase additional large transfer tanks to quickly build a Western Blot transfer platform using the Mini PROTEANTetra electrophoresis tank foundation, achieving seamless connection from separation to transfer.

This article will systematically sort out the core information of the Bole electrophoresis three piece transfer printing system from the dimensions of product composition, technical specifications, collaboration mechanism, selection comparison, and daily maintenance, to help the laboratory establish a clear reference framework in equipment selection and use.

1、 First, let's take a look at the overall positioning: one platform, two sets of functions

Before delving into the technical details, let's summarize the core design concept of the Bole electrophoresis three piece transfer system in one sentence: using the Mini PROTEANTetra electrophoresis tank as the universal platform base, through interchangeable functional modules, vertical electrophoresis and wet transfer can be achieved separately, and combined with the PowerPac Basic basic power supply to provide unified electrical parameter control.

The 1658033 package is not a simple packaging of independent devices, but an integrated solution that combines interfaces and functions. In terms of specific composition, the Mini PROTEANTetra small vertical electrophoresis tank (item number 1658001) serves as the core skeleton of the system, providing a buffer tank, tank cover, and electrode interface; MiniTrans BlotModule electrophoretic transfer core module (item number 1703935) can replace the electrophoretic module and be loaded into the same buffer tank, converting the electrophoretic tank into a transfer tank; The PowerPacBasic basic power supply (item number 1645050) provides stable and programmable power output for both electrophoresis and transfer printing processes.

The design advantage of this system lies in modular interchangeability. The interchangeable modules enable the Mini PROTEANTetra electrophoresis cell to switch roles between different applications. Each module can be matched with the same buffer tank and cover to form a complete electrophoresis tank. Therefore, with only one set of power supply and buffer tank base, the entire process from protein separation to membrane transfer printing can be covered, saving laboratory space and reducing equipment procurement and management costs.

Mini PROTEANTetra electrophoresis tank: a multifunctional platform at the core of the system

Core flux: up to four gel electrophoresis can be completed within one hour

The electrophoresis tank is the core carrier of the entire three piece set. The outstanding advantage of the Mini PROTEANTetra electrophoresis tank in the Bole electrophoresis three piece transfer printing system is its flexible flux. The electrophoresis tank can accommodate 1 to 4 pieces of hand filled gel or ReadyGel pre made gel, and experimenters can flexibly choose according to their research needs. Under conventional SDS-PAGE conditions (200V constant pressure), the typical running time is 35 to 45 minutes, supporting electrophoresis of up to four small gels within one hour.

Gel specification and compatibility

In terms of gel configuration, the electrophoresis tank is compatible with both hand gel and preformed gel. The gel size of preformed glue is 8.6 × 6.8cm, and Mini PROTEAN preformed glue system is used; The gel size of hand glue is 8.3 × 7.3 cm, which is poured with Mini PROTEAN glass plate. The glass panel specifications are unified as short glass panel 10.1 × 7.3cm, and long glass panel with edge banding 10.1 × 8.2cm.

The edge sealing strip is fixed on the long glass plate, and the advantage of this design is to ensure precise alignment of the glass plate, prevent glue leakage, and save the tedious step of manually installing the strip before each glue making process. The rubber making frame with simple cam ensures accurate alignment on any horizontal plane. The parallel glue filling rack can fill two gel at the same time. The spring lever design makes the soft rubber gasket have good sealing performance.

Buffer volume and operating parameters

The total volume of buffer solution for running 2 gels is 700mL, and for running 4 gels it is 1000mL. Typical SDS-PAGE operating conditions are 35 to 45 minutes at a constant pressure of 200V. The recommended power supply is PowerPacBasic or PowerPacHC. The overall dimensions of the electrophoresis tank are 12 × 16 × 18cm, with a weight of approximately 1.0kg, making it a compact desktop design.

Sample assisted design

The electrophoresis tank is equipped with a sample guiding device, which can prevent the lane from missing samples or repeating samples. This design is more practical in porous comb and high-throughput operations. The special plastic sampling comb will not inhibit the gel polymerization reaction. The built-in ridge in the rubber making process can prevent the gel from contacting with the air and ensure the uniform gel polymerization. Glass plates and sample combs labeled with thickness and number of holes are also convenient for quick identification and retrieval.

MiniTrans Blot transfer module: protein transfer can be completed on the same platform

Module compatibility design: Transform electrophoresis tank into transfer tank

The other core component of the Bole electrophoresis three piece transfer printing system is the MiniTrans BlotModule electrophoresis transfer core module (item number 1703935). The design concept of this module is to enable users to quickly set up a Western Blot transfer platform on the same buffer tank by simply removing and replacing the electrophoresis module in the electrophoresis tank with a transfer core module, without the need to purchase an independent transfer tank.

The small Trans Blot transfer tank can be used as a complete standalone device or as a module compatible with Mini PROTEANTetra electrophoresis tanks. This "one slot dual-use" design significantly reduces equipment investment costs and simplifies the experimental process.

Transfer efficiency: transfer 2 gel within 1 hour

In terms of transfer speed, the system can transfer two 7.5 × 10cm gel in one hour. The electrode wires of the transfer groove are 4cm apart, which can generate a strong electric field strength to ensure effective protein transfer. At the same time, the system is equipped with a built-in Bio Ice cooling device, which can quickly absorb the heat generated during the transfer printing process, reduce the interference of thermal effects on protein activity, and also allow low-intensity overnight transfer printing when needed.

Structural details and operational convenience

The transfer tank can accommodate two gel holder transfer clips, which are used to electrotransfer two small gel, namely, Mini PROTEAN3 gel and ReadyGel preform glue, in the Mini PROTEAN3 electrophoresis tank. The transfer clip and electrode are color marked to ensure the correct orientation of the gel during the transfer process and prevent the operator from misdirecting the protein transfer due to directional packaging.

The maximum gel size of the transfer tank is 10 × 7.5cm, the buffer solution is required to be 450mL, and the volume is 12 × 16 × 18cm. The electrode is of plate design, the cathode is made of titanium coated with platinum, and the anode is made of stainless steel, which produces a higher electric field than the ordinary electrode. The hinge design of the transfer clip can prevent the gel from sliding and facilitate the assembly of the transfer clip.

PowerPacBasic Basic Power Supply: Unified Control of Power Parameters for Separation and Transfer Printing

Programmable output range, covering two application requirements

The third major component of the Bole electrophoresis three piece transfer printing system is the PowerPac Basic basic power supply (item number 1645050). SDS-PAGE electrophoresis typically requires a constant voltage output of around 200V, while wet transfer printing requires lower voltage and moderate current. The output range of PowerPacBasic can meet both of these requirements: voltage from 10 to 300V, with a minimum adjustable unit of 1V; current from 4 to 400mA, with a minimum adjustable unit of 1mA; and maximum power of 75W.

In terms of output type, it supports both constant voltage and constant current modes, with pause/continue function and automatic recovery function after power failure, and can be timed for 1 to 999 minutes. The output sockets are 4 pairs in parallel, which can simultaneously perform electrophoresis on four electrophoresis tanks of the same type. This multi slot parallel capability enables one power supply to support all electrophoresis and transfer tasks of the entire three piece set.

Safety and Compliance

In terms of security protection, PowerPacBasic has passed EN-61010 and CE standard certification, and has multiple safety functions such as no-load detection, sudden load detection, overload/short circuit protection, input line protection, and power-off protection. The input voltage ranges from 100 to 120/220 to 240VAC, with automatic conversion to 50 or 60Hz. The external dimensions are 24.5 × 21 × 6.5cm, with a weight of approximately 1.1kg, compact and portable.

How is leak proof sealing achieved in the glue making and filling system?

In addition to electrophoresis and transfer printing modules, the design of the Bole electrophoresis three piece transfer printing system in the glue making process is also worth paying attention to. The leak proof sealing of the glue making system is mainly achieved through the following three mechanisms:

The soft rubber gasket forms a tight seal with the glass plate to ensure no leakage of the sealant. The edge banding strip is fixed on the long glass plate to ensure precise alignment of the glass plate. The potting frame with a simple cam ensures precise alignment on any plane, and the spring lever design provides excellent sealing for soft rubber precipitation.

These three mechanisms work together to effectively reduce the probability of glue leakage through triple protection, from aligning glass plates, mechanical compression to soft cushion sealing. The parallel glue filling rack supports the simultaneous filling of two gel, which improves the efficiency of glue making.

2、 Application scenarios: What experiments is this system suitable for?

The application positioning of the 1658033 set is a routine experimental scenario for small vertical electrophoresis, especially suitable for the following research directions:

In terms of conventional protein separation, it is used for polyacrylamide gel electrophoresis, including SDS-PAGE denaturing electrophoresis and native PAGE natural electrophoresis, to meet the basic needs of protein molecular weight determination, purity detection and quantitative analysis.

In terms of Western Blot transfer printing, the entire process from separation to transfer printing is completed on the same platform, suitable for protein immunoblotting detection. The system design allows the completion of two-dimensional gel electrophoresis in one day, which is used for exploratory research, screening new samples and evaluating sample preparation conditions.

In terms of high throughput requirements, electrophoresis of up to four gel can be completed within one hour, which is suitable for experiments requiring rapid screening of samples or optimization of conditions. When two electrophoresis modules are used, the electrophoresis tank can conduct electrophoresis of four gel at the same time.

Selection comparison: Differences between the 1658033 set and other Bio Rad transfer printing systems

After understanding the basic composition of 1658033, it is necessary to make a brief comparison with other transfer printing systems under Bio Rad in order to make a more reasonable judgment when selecting.

1658033 (Basic Set): Classic Wet Conversion Solution

The package adopts wet transfer method, which requires 450mL transfer buffer solution, and the transfer time is one hour to complete two gel. The set includes three core components: electrophoresis tank, transfer core, and basic power supply, suitable for conventional laboratories with relatively limited budgets and no need for extremely fast transfer speeds.

Trans BlotTurbo (Fast Semi Dry Rotary System)

The Turbo system adopts semi dry transfer printing technology, which significantly improves the transfer speed and completes the transfer of 2 TGX small glues within 3 minutes, and completes the transfer of 4 ordinary small glues or 2 medium-sized glues within 7 minutes. The flux is 4 pieces of small glue or 2 pieces of medium glue. The significant feature of this system is the integrated power supply design, which does not require additional power supply and does not require buffer solution during operation. The pre assembled imprint clip contains buffer solution and does not require the preparation of transfer liquid and transfer film.

Trans BlotSD (semi dry transfer printing tank)

The SD system belongs to the semi dry transfer tank and does not require a buffer tank or transfer clamp. It can complete transfer within 15 to 60 minutes and is positioned as a fast, economical, and efficient semi dry transfer solution. Suitable for laboratories with certain requirements for transfer speed but insufficient budget to purchase Turbo systems.

How to choose?

In short, if the daily Western Blot sample size in the laboratory is not large and the requirements for transfer speed are not strict, the 1658033 set is a cost-effective entry-level choice that covers the entire process of electrophoresis and wet transfer. If you pursue a convenient experience of ultra fast transfer printing and no need to prepare buffer solutions, Trans BlotTurbo is an efficient solution. If only an economical semi dry transfer printing device is needed, Trans BlotSD can meet the basic requirements.

3、 Daily maintenance and usage precautions

In terms of cleaning process, after each use, the electrophoresis tank and transfer tank should be rinsed with deionized water to remove residual salt from the electrophoresis buffer and transfer solution, including the buffer tank, tank cover, glue frame, and transfer clamp. These salts will crystallize and adhere after drying up, affecting the sealing and electric field uniformity of the next experiment.

Inspection of edge banding strips, regularly check whether the edge banding strips on the long glass plate have aged, cracked or deformed. If the elasticity of the gasket decreases or the sealing is not tight, the corresponding glass panel component should be replaced in a timely manner, otherwise the problem of glue leakage will repeatedly occur.

The electrode of the transfer core is clean. The plate electrode of the transfer core (the cathode is made of platinum coated titanium and the anode is made of stainless steel) should be gently wiped after each transfer to remove gel fragments and protein residues that may be attached. If there are obvious stains or corrosion spots on the electrode surface, it is necessary to contact the after-sales service in a timely manner to evaluate whether replacement is needed.

The standardization of power supply usage should follow the sequence of "turn on the power first, then set, and then turn off the switch" during operation. Do not replace the connection wires of the electrophoresis or transfer tank while it is powered on. PowerPacBasic supports timing from 1 to 999 minutes. It is recommended to set the timing during transfer to avoid prolonged unattended operation.

summary

The core value of Bole Electrophoresis Three piece Transfer System 1658033 is to integrate vertical electrophoresis and wet transfer on a unified platform through modular exchange design, so as to achieve an efficient workflow of four gel electrophoresis in one hour and two gel wet transfer in one hour.

Among them, the Mini PROTEANTetra electrophoresis tank serves as a universal platform, supporting flexible configuration of 1 to 4 hand filled or pre made gels, and has multiple leak proof designs such as edge sealing pad fixation, precise alignment of cam made gel frames, and spring lever sealing. The MiniTrans Blot transfer core module can be loaded into the same buffer tank, equipped with a 4cm electrode spacing to generate a strong electric field, and combined with an internal cooling device to absorb heat. The PowerPac Basic basic power supply provides programmable outputs of 10 to 300V and 4 to 400mA, meeting the dual needs of electrophoresis and transfer printing.

In terms of selection, the 1658033 set is suitable for conventional Western Blot experiments and laboratories with moderate budgets; Trans BlotTurbo and Trans BlotSD are respectively positioned for ultra fast semi dry transfer printing and economical semi dry transfer printing scenarios. No matter which configuration is chosen, standardized operation, regular cleaning and maintenance, and attention to the edge banding and electrode status are the basic guarantees for ensuring the long-term stable operation of the system.

The selection of laboratory electrophoresis and transfer printing equipment directly affects the efficiency and reproducibility of Western Blot experiments. Understanding the modular design logic of the Bole electrophoresis three piece transfer printing system and the collaborative mechanism between its components can help to use this equipment more efficiently in daily experiments and provide a clear reference direction for upgrading transfer printing solutions in the future.

Bole electrophoresis three piece transfer printing system 2026 priceHangzhou Qiannuo Biotechnology can inquire about spot prices online at any time.