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Shanghai Qifa Biotechnology Co., Ltd
Ethanol oxidase, ≥ 10U/mg
NegotiableUpdate on 05/06
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Overview
Alcohol oxidase (AOX, EC 1.1.3.15) is an oxidoreductase that catalyzes the reaction of ethanol with oxygen to produce acetaldehyde and *. This enzyme is a key enzyme in the methanol metabolism pathway, with high specificity for short chain primary alcohols such as methanol and ethanol. Due to its reaction generating *, it is widely used in fields such as biosensors, reagent kits, food analysis, and biocatalysis as an effective tool for detecting ethanol or oxygen consumption.
Product Details
Enzyme activity:≥10 units/mg solid (干粉)
Protein content:According to the Lowry method, the protein content in the solid is ≥ 90% (example, can be modified based on actual data)
AppearanceWhite to off white freeze-dried powder or microspheres
Optimal pH:7.0-8.0 (phosphate buffer solution)
Optimal temperature:30 - 40°C
PH stability:PH 6.0-8.5 (25 ° C, 24 hours)
Thermal stability:Stable at ≤ 40 ° C (pH 7.5, 30 minutes)
Molecular weight:Approximately 670-700 kDa (composed of 8 subunits of approximately 80 kDa)
Substrate specificity:Ethanol oxidase, ≥ 10U/mgIt has the highest activity towards methanol and ethanol, and can also act on other short chain primary alcohols, but with lower activity. Almost does not affect secondary and tertiary alcohols.
Inhibitors:Heavy metal ions (such as Ag ⁺, Cu ² ⁺, Hg ² ⁺), thiol reagents, and p-hydroxymercurobenzoic acid can inhibit enzyme activity.
Stabilizers:Bovine serum albumin (BSA) or carrier protein can be used to stabilize diluted enzyme solutions.
