Non contact ultrasonic cell disruptorIt is an experimental device that achieves cell lysis based on ultrasonic cavitation effect, mechanical effect, and thermal effect. It is designed with a closed cup-shaped container that allows for contactless sample processing through centrifuge tubes, and is widely used in fields such as biomedicine (vaccine preparation, genomics research), food testing, environmental science, and agricultural research.
The main applications of non-contact ultrasonic cell disruptor (cell disruptor) are:
1. Drug extraction, cell, bacterial, and viral tissue fragmentation. Extraction of intracellular contents.
2. The dispersion, homogenization, and emulsification of material particles. Such as the dispersion of nanomaterials.
3. Accelerate dissolution and chemical reactions. If used for chemical synthesis, this ultrasonic equipment is equipped with a soundproof box bracket, soundproof box, and low-temperature system.
To determine whether the cell lysis effect is good, it is necessary to combine multiple detection methods:
Microscopic observation method:
Under a high-power microscope, when the cell membrane of most cells ruptures, intracellular substances are released, and the size of cell fragments is relatively uniform without a large amount of intact cells remaining, it can be considered that the fragmentation effect is good. At this time, the cell fragmentation rate can usually reach 80% -90%.
Flow cytometry detection:
When the proportion of cells with intact cell membranes in the cell population is detected to be less than 15% -20%, and the fragmented cell fragments conform to the expected characteristics in the scattered light signal distribution, it means that the fragmentation effect is good.
Enzyme activity assay:
By measuring enzyme activity after cell lysis, it can be determined that the degree of fragmentation is appropriate when the activity recovery rate of the target enzyme is between 60% and 70% and the enzyme activity remains stable.
Conductivity variation:
After cell lysis, the conductivity of the suspension reaches a relatively stable value, which is consistent with the reported range of conductivity for similar cell lysis in literature (e.g. between 2-4mS/cm), indicating that the degree of fragmentation has reached a good state.
Particle size distribution evaluation:
Through laser particle size analyzer detection, the particle size of fragmented cells is mainly concentrated in a specific range (such as 0.5-2 µ m), and the particle size distribution is relatively narrow, indicating good uniformity of fragmentation.