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Working principle and main parameters of non-contact ultrasonic DNA disruptor
Date: 2025-11-08Read: 3

Traditional probesUltrasonic disruptorThe probe is in direct contact with the sample and can only process one sample at a time, resulting in a long experimental period; For multiple samples, it is necessary to reuse the same probe, which can easily cause cross contamination of the samples. Due to the different depths at which the probe is inserted into the sample, the energy distribution of each ultrasound is also different, which affects the repeatability and accuracy of the experimental results. In addition, since the closed system cannot be used, the aerosol or foam generated in the ultrasonic process will diffuse to the environment, causing potential biological hazards.

Main parameters:
1. Control method:
1) Ultrasonic sample volume: With different adapters, the volume of ultrasonic samples can reach 0.1ml or more or 5ul or less
2) Applicable sample types: Applicable sample types include 0ml, 0.2ml, 5ml, and 2ml centrifuge tubes, which do not require the use of special materials (such as glass) consumables, saving experimental costs
3) The sample needs to be crushed in a closed container without producing infectious flying mist, and no additional ultrasonic probe needs to be inserted
4) Allowable single operation quantity: 24 samples can be processed at a time (requires 0.1/0.2ml adapter)
5) The sample needs to be able to automatically rotate at a constant speed during ultrasound to ensure consistent crushing effect
2. Sample fragmentation method: Use magnetic resonance ultrasound technology to crush samples, with nucleic acid samples fragmented in sizes ranging from 1kb to 200bp or smaller
3. Ultrasonic control:
Working mode: 7-inch touch screen touch operation, simple and convenient operation; Real time display of working parameters on the screen and cumulative display of operating status; The microprocessor controller can store more than 20 sets of work programs, and the 99 hour process control timer controls the total time from 1 second to 99 hours, 99 minutes, and 99 seconds, meeting different experimental needs
4. The single sink digital timer has overheat and power-off protection function, and the ultrasonic power is infinitely adjustable with a step of one percent. The power applied to the sample is adjustable from 1-500W, suitable for experiments with different samples. The ultrasonic power can be adjusted within a wide range, meeting more experimental requirements
5. Ultrasound can work continuously or intermittently
6. Equipped with overheat protection function
7. Transducer size: 1313cm circular, energy gathering type generates ultrasonic energy
8. Ultrasonic water tank volume ≥ 1500mL
9. It can be connected to an external MINI chiller to cool down the ultrasonic tank, and can also work at high temperatures to ensure the required temperature environment for the sample
10. The sample needs to be crushed in a closed container without producing infectious flying mist, and no additional ultrasonic probe needs to be inserted
11. The sample automatically rotates at a constant speed during ultrasound to ensure consistent fragmentation effect
12. Allowable single operation quantity: 24 samples can be processed at once (with 0.1/0.2ml adapter)
13. The instrument contains various adapters such as 0.1ml, 0.2ml, 0.5ml, 5ml, and 2ml