The inert gas inside the glove box is circulated in a closed manner through a circulating fan and purifier, continuously removing water and oxygen

1Vacuum pumping and inflation: First, open the door inside the transition chamber, close the glove interface cover, valve, and the door outside the transition chamber. Open the three-way valve between the glove ports, so that both the inside and outside of the glove can be evacuated simultaneously during pumping, maintaining a balance of air pressure on both sides. Connect the vacuum pump to a valve in the transition chamber, turn on the vacuum pump, slowly open this valve, and evacuate the system. When the pointer of the vacuum gauge drops and stabilizes at -0.1MPa, the vacuum pumping is completed. At this time, the valve should be closed first and then the vacuum pump should be turned off. Then, inert gas is filled into the box through another valve until the pressure is slightly higher than atmospheric pressure, ensuring that the atmosphere does not seep into the inside of the box（Repeat three times).

2. Place the object into the main box: After completing the vacuuming and inflation operations mentioned above, first close the inner door of the transition chamber, open the outer door of the transition chamber, place the object in, then close the outer door, and use the two valves on the transition chamber for vacuuming and inflation. It is necessary to ensure that the pressure in the main box and transition chamber is basically balanced with atmospheric pressure before opening the doors inside and taking objects into the main box.

3. Remove the object from the main box: First, place the object into the transition chamber and close the door inside the transition chamber tightly. If the air pressure inside the transition chamber is basically balanced with atmospheric pressure, the door outside the transition chamber can be opened to remove the object. Whether opening the inner or outer door, it is necessary to ensure that the air pressure on both sides of the door is basically balanced. Otherwise, it will either fail to open or cause an explosion.

4. Precautions for operation: Before the experiment, a comprehensive and thorough plan must be made, taking into account the specific operations, instruments used, feeding sequence, post-treatment methods, etc. for each step of the experiment. In operation, it is necessary to be strict, meticulous, quick in action, and operate correctly. During the experiment, you should first move your head and start with your hands