Traditional stainless steel chromatography columns are prone to peak tailing and decreased sensitivity when analyzing metal sensitive compounds such as sulfur-containing, nitrogen-containing, phosphate compounds, and acidic polar substances due to non-specific adsorption (NSA) on the metal surface.Inert biphenyl columnBy using chemical vapor deposition (CVD) technology to form organic-inorganic hybrid coatings on the inner walls of column tubes, the interaction between analytes and metal surfaces is isolated, thereby eliminating NSA issues.

Structure and principle: The stationary phase of the inert biphenyl column is biphenyl groups, bonded to a silica gel matrix. The benzene ring in the biphenyl group can form π - π interactions with the analyte molecule, and its unique spatial structure has molecular recognition ability, which makes the inert biphenyl column effective in separating aromatic compounds and their isomers.

Performance advantage: It has higher selectivity and retention ability for compounds that are difficult to separate or elute early in C18 and other phenyl phases. At the same time, it can reduce ionization inhibition phenomena and use a simple and suitable mobile phase for mass spectrometry (MS) analysis, improving sensitivity and selectivity in LC-MS analysis.

Application areas: Inert biphenyl columns are suitable for compound analysis in various fields. In biological analysis, it is particularly suitable for drug and metabolite analysis, such as the analysis of steroids and their phase II metabolites. In pesticide analysis, metal sensitive compounds such as organophosphorus pesticides can be effectively separated and detected. In addition, in fungal toxin analysis, the analysis process can also be simplified and peak shape can be improved.