Inert biphenyl columnIt is a high-performance liquid chromatography column commonly used for separating and analyzing complex compounds. The technological breakthroughs include:

1. No need for passivation treatment: Traditional methods require reducing adsorption through acidic passivation or mobile phase additives, but this may decrease system sensitivity or alter retention time. Inert biphenyl column can be directly used to simplify the operation process.

2. Peak shape and response improvement: Experimental data shows that the peak area and peak height of inert columns are significantly improved compared to traditional stainless steel columns.

3. High throughput stability: The coating process ensures consistent performance during long-term use, reduces equilibration time, and is suitable for large-scale sample analysis.

　　Inert biphenyl columnOperation and maintenance:

1. Sample pretreatment:

Polar compounds: It is recommended to remove matrix interference through solid-phase extraction (SPE) or liquid-liquid extraction (LLE).

Metal sensitive compounds: Avoid using metal needles or containers to reduce metal contact during pre-treatment.

2. Optimization of mobile phase:

Initial conditions: methanol water (70:30, v/v), containing 0.1% formic acid (to improve peak shape).

Gradient elution: Adjust the polarity of the compound to avoid coating damage caused by high salt concentrations (such as>10 mM buffer salts).

3. Cleaning and Preservation:

Daily cleaning: Rinse with methanol for 30 minutes to remove residual analytes.

Long term storage: Fill the column with methanol, seal both ends, and store in a cool and dry place.